7UV5

The crystal structure of Papain-Like Protease of SARS CoV-2, C111S/D286N mutant, in complex with a Lys48-linked di-ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Papain-like protease nsp3	polymer	318	35926.7	1	UniProt (P0DTC1) Pfam (PF08715) UniProt (by SIFTS) (P0DTD1) In PDB	Severe acute respiratory syndrome coronavirus 2	Non-structural protein 3,nsp3,PL2-PRO,Papain-like proteinase,PL-PRO
2	B	Ubiquitin	polymer	76	8576.8	1	UniProt (P0CG48) Pfam (PF00240) In PDB	Homo sapiens (human)
3	C	Ubiquitin	polymer	76	8604.8	1	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (human)
4	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	A, B	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
6		water	water		18.0	355	Chemie (HOH)

Sequence modifications

A: 1 - 315 (UniProt: P0DTC1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag
Ser 111	Cys 1674	engineered mutation
Asn 286	Asp 1849	engineered mutation

C: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Arg 48	Lys 48	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	53108.4
Non-Polymers*	Number of molecules	5
	Total formula weight	313.7
All*	Total formula weight	53422.1

*Water molecules are not included.