7UTD
The 2.19-angstrom CryoEM structure of the [NiFe]-hydrogenase Huc from Mycobacterium smegmatis - Complex minus stalk
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G, I... (A, C, E, G, I...) | Hydrogenase-2, large subunit | polymer | 513 | 57217.1 | 8 | UniProt (A0QUM7) Pfam (PF00374) | Mycolicibacterium smegmatis MC2 155 | |
| 2 | B, D, F, H, J... (B, D, F, H, J...) | Hydrogenase-2, small subunit | polymer | 369 | 39659.8 | 8 | UniProt (I7G634) Pfam (PF01058) Pfam (PF14720) UniProt (by SIFTS) (A0QUM6) | Mycolicibacterium smegmatis MC2 155 | NADH ubiquinone oxidoreductase 20 kDa subunit |
| 3 | Q, R, S, T (Q, R, S, T) | Type 2 [NiFe]-hydrogenase Huc membrane adapter subunit | polymer | 60 | 6416.3 | 4 | UniProt (A0QUM5) | Mycolicibacterium smegmatis MC2 155 | |
| 4 | BA, DB, IA, KB, PA... (C, K, E, M, G...) | NICKEL (III) ION | non-polymer | 58.7 | 8 | Chemie (3NI) | |||
| 5 | CA, EB, JA, LB, QA... (C, K, E, M, G...) | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 8 | Chemie (FCO) | |||
| 6 | DA, FB, KA, MB, RA... (C, K, E, M, G...) | MAGNESIUM ION | non-polymer | 24.3 | 8 | Chemie (MG) | |||
| 7 | EA, GB, LA, NB, SA... (D, L, F, N, H...) | MENAQUINONE-9 | non-polymer | 785.2 | 8 | Chemie (MQ9) | |||
| 8 | AA, AB, BB, CB, FA... (B, J, D, L, F...) | FE3-S4 CLUSTER | non-polymer | 295.8 | 24 | Chemie (F3S) |
Sequence modifications
A, C, E, G, I, K, M, O: 4 - 516 (UniProt: A0QUM7)
B, D, F, H, J, L, N, P: 2 - 323 (UniProt: I7G634)
| PDB | External Database | Details |
|---|---|---|
| Dhi 166 | His 166 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -45 | - | initiating methionine |
| Ser -44 | - | expression tag |
| Ala -43 | - | expression tag |
| Trp -42 | - | expression tag |
| Ser -41 | - | expression tag |
| His -40 | - | expression tag |
| Pro -39 | - | expression tag |
| Gln -38 | - | expression tag |
| Phe -37 | - | expression tag |
| Glu -36 | - | expression tag |
| Lys -35 | - | expression tag |
| Gly -34 | - | expression tag |
| Gly -33 | - | expression tag |
| Gly -32 | - | expression tag |
| Ser -31 | - | expression tag |
| Gly -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Gly -28 | - | expression tag |
| Ser -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Ser -24 | - | expression tag |
| Ala -23 | - | expression tag |
| Trp -22 | - | expression tag |
| Ser -21 | - | expression tag |
| His -20 | - | expression tag |
| Pro -19 | - | expression tag |
| Gln -18 | - | expression tag |
| Phe -17 | - | expression tag |
| Glu -16 | - | expression tag |
| Lys -15 | - | expression tag |
| Ser -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 20 |
| Total formula weight | 800679.8 | |
| Non-Polymers* | Number of molecules | 56 |
| Total formula weight | 15132.0 | |
| All* | Total formula weight | 815811.9 |
