7UPX

Three RBD-down state of SARS-CoV-2 D614G spike in complex with the SP1-77 neutralizing antibody Fab fragment (local refinement of the RBD and Fab variable domains)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Spike glycoprotein	polymer	1310	144858.0	1	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601)	Severe acute respiratory syndrome coronavirus	S glycoprotein,E2,Peplomer protein
2	B (H)	SP1-77 Fab heavy chain	polymer	451	49665.7	1		Homo sapiens (Human)
3	C (L)	SP1-77 Fab light chain	polymer	213	23294.6	1		Homo sapiens (Human)
4	D (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)

Sequence modifications

A: 1 - 1273 (UniProt: P0DTC2)

PDB	External Database	Details
Gly 614	Asp 614	engineered mutation
Leu 1274	-	expression tag
Glu 1275	-	expression tag
Ser 1276	-	expression tag
Gly 1277	-	expression tag
Gly 1278	-	expression tag
Gly 1279	-	expression tag
Ser 1280	-	expression tag
Ala 1281	-	expression tag
Trp 1282	-	expression tag
Ser 1283	-	expression tag
His 1284	-	expression tag
Pro 1285	-	expression tag
Gln 1286	-	expression tag
Phe 1287	-	expression tag
Glu 1288	-	expression tag
Lys 1289	-	expression tag
Gly 1290	-	expression tag
Gly 1291	-	expression tag
Gly 1292	-	expression tag
Ser 1293	-	expression tag
Gly 1294	-	expression tag
Gly 1295	-	expression tag
Gly 1296	-	expression tag
Ser 1297	-	expression tag
Gly 1298	-	expression tag
Gly 1299	-	expression tag
Ser 1300	-	expression tag
Ser 1301	-	expression tag
Ala 1302	-	expression tag
Trp 1303	-	expression tag
Ser 1304	-	expression tag
His 1305	-	expression tag
Pro 1306	-	expression tag
Gln 1307	-	expression tag
Phe 1308	-	expression tag
Glu 1309	-	expression tag
Lys 1310	-	expression tag

H: 1 - 94 (PDB: 7UPX)
H: 95 - 102 (PDB: 7UPX)
H: 103 - 440 (PDB: 7UPX)
L: 1 - 97 (PDB: 7UPX)
L: 98 - 108 (PDB: 7UPX)
L: 109 - 214 (PDB: 7UPX)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	217818.4
Branched	Number of molecules	1
	Total formula weight	570.5
All*	Total formula weight	218388.9

*Water molecules are not included.