7UPI
Cryo-EM structure of SHOC2-PP1c-MRAS holophosphatase complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ras-related protein M-Ras | polymer | 183 | 20894.9 | 1 | UniProt (O14807) Pfam (PF00071) | Homo sapiens (human) | Ras-related protein R-Ras3 |
| 2 | B (B) | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit | polymer | 331 | 37615.1 | 1 | UniProt (P62136) Pfam (PF16891) Pfam (PF00149) | Homo sapiens (human) | PP-1A |
| 3 | C (C) | Leucine-rich repeat protein SHOC-2 | polymer | 583 | 65029.9 | 1 | UniProt (Q9UQ13) Pfam (PF13855) | Homo sapiens (human) | Protein soc-2 homolog,Protein sur-8 homolog |
| 4 | D (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 5 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 6 | F, G (B) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 7 | H (B) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) |
Sequence modifications
A: 1 - 182 (UniProt: O14807)
B: 1 - 330 (UniProt: P62136)
C: 1 - 582 (UniProt: Q9UQ13)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Leu 71 | Gln 71 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 123539.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 692.8 | |
| All* | Total formula weight | 124232.7 |
