7UNF
CryoEM structure of a mEAK7 bound human V-ATPase complex
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (a) | V-type proton ATPase 116 kDa subunit a 4 | polymer | 856 | 98530.5 | 1 | UniProt (Q9HBG4) Pfam (PF01496) | Homo sapiens (human) | V-ATPase 116 kDa isoform a 4,Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4,Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform |
| 2 | B (U) | KIAA1609 protein, isoform CRA_a | polymer | 456 | 51096.5 | 1 | UniProt (D3DUL8) Pfam (PF07534) UniProt (by SIFTS) (Q6P9B6) | Homo sapiens (human) | |
| 3 | C, D, E (L, M, N) | V-type proton ATPase catalytic subunit A | polymer | 617 | 68379.9 | 3 | UniProt (P38606) Pfam (PF02874) Pfam (PF16886) Pfam (PF00006) Pfam (PF22919) | Homo sapiens (human) | V-ATPase subunit A,V-ATPase 69 kDa subunit,Vacuolar ATPase isoform VA68,Vacuolar proton pump subunit alpha |
| 4 | F, G, H (O, P, Q) | V-type proton ATPase subunit B, brain isoform | polymer | 511 | 56561.5 | 3 | UniProt (P21281) Pfam (PF02874) Pfam (PF00006) Pfam (PF22919) | Homo sapiens (human) | V-ATPase subunit B 2,Endomembrane proton pump 58 kDa subunit,HO57,Vacuolar proton pump subunit B 2 |
| 5 | I (D) | V-type proton ATPase subunit D | polymer | 247 | 28311.9 | 1 | UniProt (Q9Y5K8) Pfam (PF01813) | Homo sapiens (human) | V-ATPase subunit D,V-ATPase 28 kDa accessory protein,Vacuolar proton pump subunit D |
| 6 | J, K, L (b, c, d) | V-type proton ATPase subunit E 1 | polymer | 226 | 26183.3 | 3 | UniProt (P36543) Pfam (PF01991) | Homo sapiens (human) | V-ATPase subunit E 1,V-ATPase 31 kDa subunit,p31,Vacuolar proton pump subunit E 1 |
| 7 | M, N, O (e, f, g) | V-type proton ATPase subunit G 1 | polymer | 118 | 13781.5 | 3 | UniProt (O75348) Pfam (PF03179) | Homo sapiens (human) | V-ATPase subunit G 1,V-ATPase 13 kDa subunit 1,Vacuolar proton pump subunit G 1,Vacuolar proton pump subunit M16 |
| 8 | P (F) | V-type proton ATPase subunit F | polymer | 119 | 13388.2 | 1 | UniProt (Q16864) Pfam (PF01990) | Homo sapiens (human) | V-ATPase subunit F,V-ATPase 14 kDa subunit,Vacuolar proton pump subunit F |
| 9 | Q (C) | V-type proton ATPase subunit C 1 | polymer | 382 | 43999.5 | 1 | UniProt (P21283) Pfam (PF03223) | Homo sapiens (human) | V-ATPase subunit C 1,Vacuolar proton pump subunit C 1 |
| 10 | R (H) | V-type proton ATPase subunit H | polymer | 483 | 55949.9 | 1 | UniProt (Q9UI12) Pfam (PF03224) Pfam (PF11698) | Homo sapiens (human) | V-ATPase subunit H,Nef-binding protein 1,NBP1,Protein VMA13 homolog,V-ATPase 50/57 kDa subunits,Vacuolar proton pump subunit H,Vacuolar proton pump subunit SFD |
| 11 | S (k) | V-type proton ATPase subunit d 1 | polymer | 351 | 40369.9 | 1 | UniProt (P61421) Pfam (PF01992) | Homo sapiens (human) | V-ATPase subunit d 1,32 kDa accessory protein,V-ATPase 40 kDa accessory protein,V-ATPase AC39 subunit,p39,Vacuolar proton pump subunit d 1 |
| 12 | T (m) | V-type proton ATPase subunit e 1 | polymer | 81 | 9380.3 | 1 | UniProt (O15342) Pfam (PF05493) | Homo sapiens (human) | V-ATPase subunit e 1,V-ATPase 9.2 kDa membrane accessory protein,V-ATPase M9.2 subunit,Vacuolar proton pump subunit e 1 |
| 13 | U (n) | Ribonuclease kappa | polymer | 137 | 15435.2 | 1 | UniProt (Q6P5S7) Pfam (PF23489) | Homo sapiens (human) | RNase kappa |
| 14 | V (s) | V-type proton ATPase subunit S1 | polymer | 470 | 52067.5 | 1 | UniProt (Q15904) Pfam (PF05827) Pfam (PF20520) | Homo sapiens (human) | V-ATPase subunit S1,Protein XAP-3,V-ATPase Ac45 subunit,V-ATPase S1 accessory protein,Vacuolar proton pump subunit S1 |
| 15 | W (r) | ATPase H(+)-transporting lysosomal accessory protein 2 | polymer | 345 | 38421.1 | 1 | UniProt (A0A1B0GVW0) Pfam (PF25294) Pfam (PF07850) | Homo sapiens (human) | ATPase H(+)-transporting lysosomal-interacting protein 2,Renin receptor,Renin/prorenin receptor |
| 16 | AA, BA, CA, DA, EA... (8, 9, 2, 3, 4...) | V-type proton ATPase 16 kDa proteolipid subunit | polymer | 155 | 15743.7 | 9 | UniProt (P27449) Pfam (PF00137) | Homo sapiens (human) | V-ATPase 16 kDa proteolipid subunit,Vacuolar proton pump 16 kDa proteolipid subunit |
| 17 | Z (1) | V-type proton ATPase 21 kDa proteolipid subunit | polymer | 205 | 21418.2 | 1 | UniProt (Q99437) Pfam (PF00137) | Homo sapiens (human) | V-ATPase 21 kDa proteolipid subunit,Vacuolar proton pump 21 kDa proteolipid subunit,hATPL |
| 18 | HA (t) | alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-3)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1397.2 | 1 | In PDB | |||
| 19 | IA (w) | alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose | branched | 342.3 | 1 | In PDB GlyTouCan (G77384RA) | |||
| 20 | JA (M) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 21 | KA, LA, MA, NA, OA... (s) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 6 | Chemie (NAG) | |||
| 22 | QA, RA, SA, TA, UA... (s, r, 8, 1) | (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate | non-polymer | 760.1 | 9 | Chemie (POV) |
Sequence modifications
a: 1 - 840 (UniProt: Q9HBG4)
| PDB | External Database | Details |
|---|---|---|
| Arg 252 | Pro 252 | conflict |
| Asp 841 | - | expression tag |
| Tyr 842 | - | expression tag |
| Lys 843 | - | expression tag |
| Asp 844 | - | expression tag |
| Asp 845 | - | expression tag |
| Asp 846 | - | expression tag |
| Asp 847 | - | expression tag |
| Lys 848 | - | expression tag |
| Asp 849 | - | expression tag |
| Tyr 850 | - | expression tag |
| Lys 851 | - | expression tag |
| Asp 852 | - | expression tag |
| Asp 853 | - | expression tag |
| Asp 854 | - | expression tag |
| Asp 855 | - | expression tag |
| Lys 856 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 33 |
| Total formula weight | 1104780.6 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1739.5 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 8595.1 | |
| All* | Total formula weight | 1115115.3 |
