7UL3
CryoEM Structure of Inactive H2R Bound to Famotidine, Nb6M, and NabFab
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Histamine H2 receptor | polymer | 400 | 44770.2 | 1 | UniProt (P25021) UniProt (A0A8B8VNB6) Pfam (PF00001) UniProt (by SIFTS) (P41145) In PDB | Homo sapiens (human) | H2R,HH2R,Gastric receptor I,kappa-type opioid receptor isoform X1 |
| 2 | C | Nanobody 6M | polymer | 131 | 14418.9 | 1 | synthetic construct | ||
| 3 | H | NabFab HC | polymer | 239 | 25684.5 | 1 | synthetic construct | ||
| 4 | L | NabFab LC | polymer | 215 | 23258.8 | 1 | synthetic construct | ||
| 5 | A | famotidine | non-polymer | 339.5 | 1 | Chemie (FO9) |
Sequence modifications
A: 1 - 199 (UniProt: P25021)
A: 202 - 245 (UniProt: A0A8B8VNB6)
A: 246 - 359 (UniProt: P25021)
| PDB | External Database | Details |
|---|---|---|
| Asp -38 | - | expression tag |
| Tyr -37 | - | expression tag |
| Lys -36 | - | expression tag |
| Asp -35 | - | expression tag |
| Asp -34 | - | expression tag |
| Asp -33 | - | expression tag |
| Asp -32 | - | expression tag |
| Ala -31 | - | expression tag |
| Met -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Gln -28 | - | expression tag |
| Pro -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Asn -25 | - | expression tag |
| Gly -24 | - | expression tag |
| Ser -23 | - | expression tag |
| Ala -22 | - | expression tag |
| Phe -21 | - | expression tag |
| Leu -20 | - | expression tag |
| Leu -19 | - | expression tag |
| Ala -18 | - | expression tag |
| Pro -17 | - | expression tag |
| Asn -16 | - | expression tag |
| Arg -15 | - | expression tag |
| Ser -14 | - | expression tag |
| His -13 | - | expression tag |
| Ala -12 | - | expression tag |
| Pro -11 | - | expression tag |
| Asp -10 | - | expression tag |
| His -9 | - | expression tag |
| Asp -8 | - | expression tag |
| Val -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Asn -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
A: 246 - 359 (UniProt: P25021)
| PDB | External Database | Details |
|---|---|---|
| Val 200 | - | linker |
| Cys 201 | - | linker |
| Leu 360 | - | expression tag |
| Glu 361 | - | expression tag |
| Val 362 | - | expression tag |
| Leu 363 | - | expression tag |
| Phe 364 | - | expression tag |
| Gln 365 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 108132.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 339.5 | |
| All* | Total formula weight | 108471.8 |
