7UK1

Complex Structure of Human Polypyrimidine Splicing Factor (PSF/SFPQ) with Murine Virus-like 30S Transcript-1 (VS30-1) Reveals Cooperative Binding of RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Splicing factor, proline- and glutamine-rich	polymer	412	47764.7	2	UniProt (P23246) Pfam (PF00076) Pfam (PF08075) In PDB	Homo sapiens (human)	100 kDa DNA-pairing protein,hPOMp100,DNA-binding p52/p100 complex,100 kDa subunit,Polypyrimidine tract-binding protein-associated-splicing factor,PSF,PTB-associated-splicing factor
2	A, B	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
3		water	water		18.0	28	Chemie (HOH)

Sequence modifications

A, B: 214 - 598 (UniProt: P23246)

PDB	External Database	Details
Met 187	-	initiating methionine
Lys 188	-	expression tag
His 189	-	expression tag
His 190	-	expression tag
His 191	-	expression tag
His 192	-	expression tag
His 193	-	expression tag
His 194	-	expression tag
Pro 195	-	expression tag
Met 196	-	expression tag
Ser 197	-	expression tag
Asp 198	-	expression tag
Tyr 199	-	expression tag
Asp 200	-	expression tag
Ile 201	-	expression tag
Pro 202	-	expression tag
Thr 203	-	expression tag
Thr 204	-	expression tag
Glu 205	-	expression tag
Asn 206	-	expression tag
Leu 207	-	expression tag
Tyr 208	-	expression tag
Phe 209	-	expression tag
Gln 210	-	expression tag
Gly 211	-	expression tag
Ala 212	-	expression tag
Met 213	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	95529.4
Non-Polymers*	Number of molecules	5
	Total formula weight	121.5
All*	Total formula weight	95650.9

*Water molecules are not included.