7UK1
Complex Structure of Human Polypyrimidine Splicing Factor (PSF/SFPQ) with Murine Virus-like 30S Transcript-1 (VS30-1) Reveals Cooperative Binding of RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-10-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.451, 64.658, 68.837 |
Unit cell angles | 90.00, 97.79, 90.00 |
Refinement procedure
Resolution | 48.870 - 2.700 |
R-factor | 0.2045 |
Rwork | 0.200 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wii |
RMSD bond length | 0.008 |
RMSD bond angle | 1.680 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.870 | 2.750 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmeas | 0.910 | |
Rpim | 0.129 | |
Number of reflections | 17804 | 728 |
<I/σ(I)> | 17.1 | |
Completeness [%] | 98.9 | |
Redundancy | 44.1 | |
CC(1/2) | 0.125 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 285.15 | 0.1 M MgCl2, 0.1 M HEPES pH 7.0, 15% (w/v),PEG4000, or 15% (w/v) PEG3500, or 20% PEG2000MME |