7UIO

Mediator-PIC Early (Composite Model)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (38-MER)	polymer	38	11666.5	1		Saccharomyces cerevisiae S288C
2	B (B)	DNA (37-MER)	polymer	37	11424.3	1		Saccharomyces cerevisiae S288C
3	C, QA (Ao, Bo)	Mediator of RNA polymerase II transcription subunit 15	polymer	1081	120432.2	2	UniProt (P19659) Pfam (PF16987) Pfam (PF18535) Pfam (PF05397)	Saccharomyces cerevisiae S288C	Autonomous replication regulatory protein 3,Basal expression activator protein 1,Defective silencing suppressor protein 4,Mediator complex subunit 15,Transcription regulatory protein GAL11,Ty insertion suppressor protein 13
4	D, RA (Ab, Bb)	Mediator of RNA polymerase II transcription subunit 2	polymer	431	47754.3	2	UniProt (Q12124) Pfam (PF11214)	Saccharomyces cerevisiae S288C	Mediator complex subunit 2
5	E, SA (Ac, Bc)	Mediator of RNA polymerase II transcription subunit 3	polymer	397	43116.0	2	UniProt (P40356) Pfam (PF11593)	Saccharomyces cerevisiae S288C	Hyper-recombination suppressor protein 1,Mediator complex subunit 3,Poly-glutamine domain protein 1
6	F, TA (An, Bn)	Mediator of RNA polymerase II transcription subunit 14	polymer	1082	123500.7	2	UniProt (P19263) Pfam (PF08638)	Saccharomyces cerevisiae S288C	Glucose repression regulatory protein 1,Mediator complex subunit 14
7	G, UA (Ae, Be)	Mediator of RNA polymerase II transcription subunit 5	polymer	1132	128918.8	2	UniProt (P53114) Pfam (PF08689)	Saccharomyces cerevisiae S288C	Mediator complex subunit 5,Negative regulator of URS2 protein 1
8	H, VA (Ap, Bp)	Mediator of RNA polymerase II transcription subunit 16	polymer	974	111423.4	2	UniProt (P32259) Pfam (PF11635) Pfam (PF20719)	Saccharomyces cerevisiae S288C	Global transcriptional regulator SIN4,Mediator complex subunit 16,SNF1 suppressor protein 4,SWI4 suppressor protein 5,Transcriptional silencing factor 3,YGP1 expression regulatory protein 1
9	I, J (GA, GB)	Regulatory protein GAL4	polymer	147	16895.7	2	UniProt (P04386) Pfam (PF00172) Pfam (PF03902)	Saccharomyces cerevisiae S288C
10	K, WA (At, Bt)	Mediator of RNA polymerase II transcription subunit 20	polymer	210	22918.1	2	UniProt (P34162) Pfam (PF08612)	Saccharomyces cerevisiae S288C	Hyper-recombination suppressor protein 2,Mediator complex subunit 20,Suppressor of RNA polymerase B 2
11	L, XA (Ag, Bg)	Mediator of RNA polymerase II transcription subunit 7	polymer	222	25616.1	2	UniProt (Q08278) Pfam (PF05983)	Saccharomyces cerevisiae S288C	Mediator complex subunit 7
12	M, YA (Ah, Bh)	Mediator of RNA polymerase II transcription subunit 8	polymer	223	25297.2	2	UniProt (P38304) Pfam (PF10232)	Saccharomyces cerevisiae S288C	Mediator complex subunit 8
13	N, ZA (Ai, Bi)	Mediator of RNA polymerase II transcription subunit 9	polymer	149	17400.4	2	UniProt (P33308) Pfam (PF07544)	Saccharomyces cerevisiae S288C	Chromosome segregation protein 2,Mediator complex subunit 9
14	AB, O (Aj, Bj)	Mediator of RNA polymerase II transcription subunit 10	polymer	157	17927.9	2	UniProt (Q06213) Pfam (PF09748)	Saccharomyces cerevisiae S288C	Mediator complex subunit 10,Negative regulator of URS2 protein 2
15	BB, P (Ak, Bk)	Mediator of RNA polymerase II transcription subunit 11	polymer	115	13324.2	2	UniProt (Q99278) Pfam (PF10280)	Saccharomyces cerevisiae S288C	Mediator complex subunit 11
16	CB, Q (Aq, Bq)	Mediator of RNA polymerase II transcription subunit 17	polymer	687	78582.8	2	UniProt (P32569) Pfam (PF10156)	Saccharomyces cerevisiae S288C	Mediator complex subunit 17,Suppressor of RNA polymerase B 4
17	DB, R (Ar, Br)	Mediator of RNA polymerase II transcription subunit 18	polymer	307	34316.5	2	UniProt (P32585) Pfam (PF09637)	Saccharomyces cerevisiae S288C	Mediator complex subunit 18,Suppressor of RNA polymerase B 5
18	EB, S (As, Bs)	Mediator of RNA polymerase II transcription subunit 19	polymer	220	24885.3	2	UniProt (P25046) Pfam (PF08633)	Saccharomyces cerevisiae S288C	Hypoxic gene repressor protein 3,Mediator complex subunit 19,Negative regulator of URS2 protein 3,SNF1 suppressor protein 7
19	FB, T (Au, Bu)	Mediator of RNA polymerase II transcription subunit 21	polymer	140	16093.4	2	UniProt (P47822) Pfam (PF11221)	Saccharomyces cerevisiae S288C	Mediator complex subunit 21,RNAPII complex component SRB7,Suppressor of RNA polymerase B 7
20	GB, U (Av, Bv)	Mediator of RNA polymerase II transcription subunit 22	polymer	120	13774.6	2	UniProt (P32570) Pfam (PF06179)	Saccharomyces cerevisiae S288C	Mediator complex subunit 22,Suppressor of RNA polymerase B 6
21	HB, V (Aw, Bw)	Mediator of RNA polymerase II transcription subunit 31	polymer	127	14747.7	2	UniProt (P38633) Pfam (PF05669)	Saccharomyces cerevisiae S288C	Hpr1 suppressor protein 1,Mediator complex subunit 31
22	IB, W (Az, Bz)	DNA-directed RNA polymerase II subunit RPB1	polymer	25	2611.7	2	UniProt (P04050) Pfam (PF05001)	Saccharomyces cerevisiae S288C	RNA polymerase II subunit B1,DNA-directed RNA polymerase III largest subunit,RNA polymerase II subunit B220
23	JB, X (AA, BA)	DNA-directed RNA polymerase II subunit RPB1	polymer	1453	162951.7	2	UniProt (P04050) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990)	Saccharomyces cerevisiae S288C	RNA polymerase II subunit 1,RNA polymerase II subunit B1,DNA-directed RNA polymerase III largest subunit,RNA polymerase II subunit B220
24	KB, Y (AB, BB)	DNA-directed RNA polymerase II subunit RPB2	polymer	1224	138937.3	2	UniProt (P08518) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561)	Saccharomyces cerevisiae S288C	RNA polymerase II subunit 2,B150,DNA-directed RNA polymerase II 140 kDa polypeptide
25	LB, Z (AC, BC)	DNA-directed RNA polymerase II subunit RPB3	polymer	318	35330.5	2	UniProt (P16370) Pfam (PF01193) Pfam (PF01000)	Saccharomyces cerevisiae S288C	RNA polymerase II subunit B3,B44.5,DNA-directed RNA polymerase II 45 kDa polypeptide
26	AA, MB (AD, BD)	DNA-directed RNA polymerase II subunit RPB4	polymer	221	25451.2	2	UniProt (P20433) Pfam (PF03874)	Saccharomyces cerevisiae S288C	RNA polymerase II subunit B4,B32,DNA-directed RNA polymerase II 32 kDa polypeptide
27	BA, NB (AE, BE)	DNA-directed RNA polymerases I, II, and III subunit RPABC1	polymer	215	25117.1	2	UniProt (P20434) Pfam (PF03871) Pfam (PF01191)	Saccharomyces cerevisiae S288C	RNA polymerases I,II,and III subunit ABC1,ABC27,DNA-directed RNA polymerases I,and III 27 kDa polypeptide
28	CA, OB (AF, BF)	DNA-directed RNA polymerases I, II, and III subunit RPABC2	polymer	155	17931.8	2	UniProt (P20435) Pfam (PF01192)	Saccharomyces cerevisiae S288C	RNA polymerases I,II,and III subunit ABC2,ABC23,DNA-directed RNA polymerases I,and III 23 kDa polypeptide
29	DA, PB (AG, BG)	DNA-directed RNA polymerase II subunit RPB7	polymer	171	19081.1	2	UniProt (P34087) Pfam (PF03876) Pfam (PF00575)	Saccharomyces cerevisiae S288C	RNA polymerase II subunit B7,B16
30	EA, QB (AH, BH)	DNA-directed RNA polymerases I, II, and III subunit RPABC3	polymer	146	16525.4	2	UniProt (P20436) Pfam (PF03870)	Saccharomyces cerevisiae S288C	RNA polymerases I,II,and III subunit ABC3,ABC14.4,ABC14.5,DNA-directed RNA polymerases I,and III 14.5 kDa polypeptide
31	FA, RB (AI, BI)	DNA-directed RNA polymerase II subunit RPB9	polymer	122	14308.2	2	UniProt (P27999) Pfam (PF02150) Pfam (PF01096)	Saccharomyces cerevisiae S288C	RNA polymerase II subunit B9,B12.6,DNA-directed RNA polymerase II 14.2 kDa polypeptide,DNA-directed RNA polymerase II subunit 9
32	GA, SB (AJ, BJ)	DNA-directed RNA polymerases I, II, and III subunit RPABC5	polymer	70	8290.7	2	UniProt (P22139) Pfam (PF01194)	Saccharomyces cerevisiae S288C	RNA polymerases I,II,and III subunit ABC5,ABC10-beta,ABC8,DNA-directed RNA polymerases I,and III 8.3 kDa polypeptide
33	HA, TB (AK, BK)	DNA-directed RNA polymerase II subunit RPB11	polymer	120	13633.5	2	UniProt (P38902) Pfam (PF13656)	Saccharomyces cerevisiae S288C	RNA polymerase II subunit B11,B13.6,DNA-directed RNA polymerase II 13.6 kDa polypeptide
34	IA, UB (AL, BL)	DNA-directed RNA polymerases I, II, and III subunit RPABC4	polymer	70	7730.0	2	UniProt (P40422) Pfam (PF03604)	Saccharomyces cerevisiae S288C	RNA polymerases I,II,and III subunit ABC4,ABC10-alpha
35	JA, VB (AM, BM)	Transcription initiation factor IIB	polymer	345	38257.3	2	UniProt (P29055) Pfam (PF08271) Pfam (PF00382)	Saccharomyces cerevisiae S288C	General transcription factor TFIIB,Transcription factor E
36	KA, WB (AP, BP)	Transcription initiation factor IIF subunit alpha	polymer	735	82320.6	2	UniProt (P41895) Pfam (PF05793)	Saccharomyces cerevisiae S288C	TFIIF-alpha,TFIIF large subunit,Transcription factor G 105 kDa subunit,P105
37	LA, XB (AQ, BQ)	Transcription initiation factor IIF subunit beta	polymer	400	46684.5	2	UniProt (P41896) Pfam (PF17683) Pfam (PF02270)	Saccharomyces cerevisiae S288C	ATP-dependent helicase TFG2,TFIIF medium subunit,TFIIF-beta,Transcription factor G 54 kDa subunit
38	MA, YB (AS, BS)	Transcription elongation factor S-II	polymer	309	34903.6	2	UniProt (P07273) Pfam (PF08711) Pfam (PF07500) Pfam (PF01096)	Saccharomyces cerevisiae S288C	DNA strand transfer protein alpha,STP-alpha,DNA strand transferase 1,Pyrimidine pathway regulatory protein 2
39	NA, ZB (Aa, Ba)	Mediator of RNA polymerase II transcription subunit 1	polymer	566	64315.0	2	UniProt (Q12321) Pfam (PF10744)	Saccharomyces cerevisiae S288C	Mediator complex subunit 1
40	AC, OA (Ad, Bd)	Mediator of RNA polymerase II transcription subunit 4	polymer	284	32244.9	2	UniProt (Q12343) Pfam (PF10018)	Saccharomyces cerevisiae S288C	Mediator complex subunit 4
41	BC, PA (Af, Bf)	Mediator of RNA polymerase II transcription subunit 6	polymer	295	32844.7	2	UniProt (P38782) Pfam (PF04934)	Saccharomyces cerevisiae S288C	Mediator complex subunit 6
42	CC, DC, EC, FC (A, B)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
43	GC (Bc)	THREONINE	non-polymer		119.1	1	Chemie (THR)
44	HC (Bc)	ALANINE	non-polymer		89.1	1	Chemie (ALA)
45	IC (Bc)	ASPARTIC ACID	non-polymer		133.1	1	Chemie (ASP)

Contents of the asymmetric unit

Polymers	Number of chains	80
	Total formula weight	3455882.7
Non-Polymers*	Number of molecules	7
	Total formula weight	603.0
All*	Total formula weight	3456485.6

*Water molecules are not included.