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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

7UG6

Cryo-EM structure of pre-60S ribosomal subunit, unmethylated G2922

This is a non-PDB format compatible entry.
Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(1)		25S rRNApolymer33961097493.91GenBank (831416132)Saccharomyces cerevisiae S288C
2B
(2)		5.8S rRNApolymer15850682.91GenBank (1489318609)Saccharomyces cerevisiae S288C
3C
(3)		5S rRNApolymer12138951.11GenBank (1329886537)Saccharomyces cerevisiae S288C
4D
(7)		60S ribosomal protein L30polymer10511430.41UniProt (P14120)
Pfam (PF01248)		Saccharomyces cerevisiae S288CL32,Large ribosomal subunit protein eL30,RP73,YL38
5E
(A)		60S ribosomal protein L2-Apolymer25427463.61UniProt (P0CX45)
Pfam (PF00181)
Pfam (PF03947)		Saccharomyces cerevisiae S288CL5,Large ribosomal subunit protein uL2-A,RP8,YL6
6F
(B)		60S ribosomal protein L3polymer38743850.81UniProt (P14126)
Pfam (PF00297)		Saccharomyces cerevisiae S288CLarge ribosomal subunit protein uL3,Maintenance of killer protein 8,RP1,Trichodermin resistance protein,YL1
7G
(C)		60S ribosomal protein L4-Apolymer36239159.11UniProt (P10664)
Pfam (PF00573)
Pfam (PF14374)		Saccharomyces cerevisiae S288CL2,Large ribosomal subunit protein uL4-A,RP2,YL2
8H
(D)		60S ribosomal protein L5polymer29733764.81UniProt (P26321)
Pfam (PF17144)
Pfam (PF14204)		Saccharomyces cerevisiae S288CL1,L1a,Large ribosomal subunit protein uL18,Ribosomal 5S RNA-binding protein,YL3
9I
(E)		60S ribosomal protein L6-Apolymer17620000.61UniProt (Q02326)
Pfam (PF01159)		Saccharomyces cerevisiae S288CL17,Large ribosomal subunit protein eL6-A,RP18,YL16
10J
(F)		60S ribosomal protein L7-Apolymer24427686.31UniProt (P05737)
Pfam (PF08079)
Pfam (PF00327)		Saccharomyces cerevisiae S288CL6,Large ribosomal subunit protein uL30-A,RP11,YL8
11K
(G)		60S ribosomal protein L8-Apolymer25628175.81UniProt (P17076)
Pfam (PF01248)		Saccharomyces cerevisiae S288CL4,L4-2,L7a-1,Large ribosomal subunit protein eL8-A,Maintenance of killer protein 7,RP6,YL5
12L
(H)		60S ribosomal protein L9-Apolymer19121605.11UniProt (P05738)
Pfam (PF00347)		Saccharomyces cerevisiae S288CL8,Large ribosomal subunit protein uL6-A,RP24,YL11
13M
(I)		Bud site selection protein 20polymer16618561.01UniProt (Q08004)
Pfam (PF12171)		Saccharomyces cerevisiae S288C
14N
(J)		60S ribosomal protein L11-Apolymer17419755.71UniProt (P0C0W9)
Pfam (PF00281)
Pfam (PF00673)		Saccharomyces cerevisiae S288CL16,Large ribosomal subunit protein uL5-A,RP39,YL22
15O
(L)		60S ribosomal protein L13-Apolymer19922604.21UniProt (Q12690)
Pfam (PF01294)		Saccharomyces cerevisiae S288CLarge ribosomal subunit protein eL13-A
16P
(M)		60S ribosomal protein L14-Apolymer13815195.11UniProt (P36105)
Pfam (PF00467)
Pfam (PF01929)		Saccharomyces cerevisiae S288CLarge ribosomal subunit protein eL14-A
17Q
(N)		60S ribosomal protein L15-Apolymer20424482.41UniProt (P05748)
Pfam (PF00827)		Saccharomyces cerevisiae S288CL13,Large ribosomal subunit protein eL15-A,RP15R,YL10,YP18
18R
(O)		60S ribosomal protein L16-Apolymer19922247.21UniProt (P26784)
Pfam (PF00572)		Saccharomyces cerevisiae S288CL13a,L21,Large ribosomal subunit protein uL13-A,RP22,YL15
19S
(P)		60S ribosomal protein L17-Apolymer18420589.51UniProt (P05740)
Pfam (PF00237)		Saccharomyces cerevisiae S288CL20A,Large ribosomal subunit protein uL22-A,YL17
20T
(Q)		60S ribosomal protein L18-Apolymer18620609.31UniProt (P0CX49)
Pfam (PF17135)		Saccharomyces cerevisiae S288CLarge ribosomal subunit protein eL18-A,RP28
21U
(R)		60S ribosomal protein L19-Apolymer18921762.31UniProt (P0CX82)
Pfam (PF01280)		Saccharomyces cerevisiae S288CL23,Large ribosomal subunit protein eL19-A,RP15L,RP33,YL14
22V
(S)		60S ribosomal protein L20-Apolymer17220478.91UniProt (P0CX23)
Pfam (PF01775)		Saccharomyces cerevisiae S288CL18a,Large ribosomal subunit protein eL20-A
23W
(T)		60S ribosomal protein L21-Apolymer16018279.31UniProt (Q02753)
Pfam (PF01157)		Saccharomyces cerevisiae S288CLarge ribosomal subunit protein eL21-A
24X
(U)		60S ribosomal protein L22-Apolymer12113711.41UniProt (P05749)
Pfam (PF01776)		Saccharomyces cerevisiae S288CL1c,Large ribosomal subunit protein eL22-A,RP4,YL31
25Y
(V)		60S ribosomal protein L23-Apolymer13714494.01UniProt (P0CX41)
Pfam (PF00238)		Saccharomyces cerevisiae S288CL17a,Large ribosomal subunit protein uL14-A,YL32
26Z
(W)		Ribosome assembly factor MRT4polymer23627098.01UniProt (P33201)
Pfam (PF00466)
Pfam (PF17777)		Saccharomyces cerevisiae S288CmRNA turnover protein 4
27AA
(X)		60S ribosomal protein L25polymer14215787.61UniProt (P04456)
Pfam (PF03939)
Pfam (PF00276)		Saccharomyces cerevisiae S288CLarge ribosomal subunit protein uL23,RP16L,YL25,YP42'
28BA
(Y)		60S ribosomal protein L26-Apolymer12714265.81UniProt (P05743)
Pfam (PF16906)
Pfam (PF00467)		Saccharomyces cerevisiae S288CL33,Large ribosomal subunit protein uL24-A,YL33
29CA
(Z)		60S ribosomal protein L27-Apolymer13615568.41UniProt (P0C2H6)
Pfam (PF01777)		Saccharomyces cerevisiae S288CLarge ribosomal subunit protein eL27-A
30DA
(a)		60S ribosomal protein L28polymer14916761.71UniProt (P02406)
Pfam (PF00828)		Saccharomyces cerevisiae S288CL27a,L29,Large ribosomal subunit protein uL15,RP44,RP62,YL24
31EA
(b)		Nucleolar GTP-binding protein 1polymer64774531.21UniProt (Q02892)
Pfam (PF17835)
Pfam (PF06858)
Pfam (PF08155)		Saccharomyces cerevisiae S288C
32FA
(c)		Ribosome biogenesis protein ALB1polymer17519322.01UniProt (P47019)
Pfam (PF09135)		Saccharomyces cerevisiae S288C
33GA
(d)		60S ribosomal protein L31-Apolymer11312980.21UniProt (P0C2H8)
Pfam (PF01198)		Saccharomyces cerevisiae S288CL34,Large ribosomal subunit protein eL31-A,YL28
34HA
(e)		60S ribosomal protein L32polymer13014809.41UniProt (P38061)
Pfam (PF01655)		Saccharomyces cerevisiae S288CLarge ribosomal subunit protein eL32
35IA
(f)		60S ribosomal protein L33-Apolymer10712177.11UniProt (P05744)
Pfam (PF01247)		Saccharomyces cerevisiae S288CL37,Large ribosomal subunit protein eL33-A,RP47,YL37
36JA
(g)		60S ribosomal protein L34-Apolymer12113673.21UniProt (P87262)
Pfam (PF01199)		Saccharomyces cerevisiae S288CLarge ribosomal subunit protein eL34-A
37KA
(h)		60S ribosomal protein L35-Apolymer12013942.61UniProt (P0CX84)
Pfam (PF00831)		Saccharomyces cerevisiae S288CLarge ribosomal subunit protein uL29-A
38LA
(i)		60S ribosomal protein L36-Apolymer10011151.31UniProt (P05745)
Pfam (PF01158)		Saccharomyces cerevisiae S288CL39,Large ribosomal subunit protein eL36-A,YL39
39MA
(j)		60S ribosomal protein L37-Apolymer889877.41UniProt (P49166)
Pfam (PF01907)		Saccharomyces cerevisiae S288CL43,Large ribosomal subunit protein eL37-A,YL35,YP55
40NA
(k)		60S ribosomal protein L38polymer788845.61UniProt (P49167)
Pfam (PF01781)		Saccharomyces cerevisiae S288CLarge ribosomal subunit protein eL38
41OA
(l)		60S ribosomal protein L39polymer516358.61UniProt (P04650)
Pfam (PF00832)		Saccharomyces cerevisiae S288CL46,Large ribosomal subunit protein eL39,YL40
42PA
(m)		Nucleolar GTP-binding protein 2polymer51959606.61UniProt (P53742)
Pfam (PF08153)
Pfam (PF01926)		Saccharomyces cerevisiae S288C
43QA
(p)		60S ribosomal protein L43-Apolymer9210113.01UniProt (P0CX25)
Pfam (PF01780)		Saccharomyces cerevisiae S288CL37a,Large ribosomal subunit protein eL43-A,YL35
44RA
(q)		60S ribosomal protein L12-Apolymer16517850.61UniProt (P0CX53)
Pfam (PF03946)
Pfam (PF00298)		Saccharomyces cerevisiae S288CL15,YL23
45SA
(r)		Ribosome biogenesis protein NSA2polymer26129786.81UniProt (P40078)
Pfam (PF01201)		Saccharomyces cerevisiae S288CNOP7-associated protein 2
46TA
(s)		Nuclear GTP-binding protein NUG1polymer52057798.71UniProt (P40010)
Pfam (PF08701)
Pfam (PF01926)		Saccharomyces cerevisiae S288CNuclear GTPase 1
47UA
(u)		Ribosome biogenesis protein RLP24polymer19924027.71UniProt (Q07915)
Pfam (PF01246)		Saccharomyces cerevisiae S288CRibosomal protein L24-like
48VA
(v)		Ribosome biogenesis protein RPF2polymer34439665.81UniProt (P36160)
Pfam (PF04427)		Saccharomyces cerevisiae S288C
49WA
(w)		Regulator of ribosome biosynthesispolymer20323001.41UniProt (Q08746)
Pfam (PF04939)		Saccharomyces cerevisiae S288C
50XA
(x)		Ribosome assembly protein 4polymer51456975.61UniProt (P25382)
Pfam (PF08154)
Pfam (PF00400)		Saccharomyces cerevisiae S288CNotchless protein homolog 1,Ribosome biogenesis factor RSA4
51YA
(y)		Eukaryotic translation initiation factor 6polymer24526476.61UniProt (Q12522)
Pfam (PF01912)		Saccharomyces cerevisiae S288CeIF-6
52ZA
(z)		UPF0642 protein YBL028Cpolymer10612435.41UniProt (P38202)
Pfam (PF10338)		Saccharomyces cerevisiae S288C
53AB
(m)		GUANOSINE-5'-DIPHOSPHATEnon-polymer443.21Chemie (GDP)
54BB
(m)		MAGNESIUM IONnon-polymer24.31Chemie (MG)
Sequence modifications
I: 1 - 166 (UniProt: Q08004)
PDBExternal DatabaseDetails
Leu 112Val 112conflict
m: 1 - 486 (UniProt: P53742)
PDBExternal DatabaseDetails
Gly 487-expression tag
Ser 488-expression tag
Asp 489-expression tag
Tyr 490-expression tag
Lys 491-expression tag
Asp 492-expression tag
Asp 493-expression tag
Asp 494-expression tag
Asp 495-expression tag
Lys 496-expression tag
Asp 497-expression tag
Tyr 498-expression tag
Lys 499-expression tag
Asp 500-expression tag
Asp 501-expression tag
Asp 502-expression tag
Asp 503-expression tag
Lys 504-expression tag
Asp 505-expression tag
Tyr 506-expression tag
Lys 507-expression tag
Asp 508-expression tag
Asp 509-expression tag
Asp 510-expression tag
Asp 511-expression tag
Lys 512-expression tag
Gly 513-expression tag
His 514-expression tag
His 515-expression tag
His 516-expression tag
His 517-expression tag
His 518-expression tag
His 519-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains52
Total formula weight2337922.5
Non-Polymers*Number of molecules2
Total formula weight467.5
All*Total formula weight2338390.0
*Water molecules are not included.

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PDB entries from 2025-06-11

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