7U5C
Cryo-EM structure of human CST bound to DNA polymerase alpha-primase in a recruitment state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA primase small subunit | polymer | 420 | 49981.0 | 1 | UniProt (P49642) Pfam (PF01896) | Homo sapiens (human) | DNA primase 49 kDa subunit,p49 |
| 2 | B (B) | DNA primase large subunit | polymer | 512 | 59092.1 | 1 | UniProt (P49643) Pfam (PF04104) | Homo sapiens (human) | DNA primase 58 kDa subunit,p58 |
| 3 | C (C) | DNA polymerase alpha catalytic subunit | polymer | 1132 | 129635.1 | 1 | UniProt (P09884) Pfam (PF03104) Pfam (PF00136) Pfam (PF08996) | Homo sapiens (human) | DNA polymerase alpha catalytic subunit p180 |
| 4 | D (D) | DNA polymerase alpha subunit B | polymer | 598 | 66015.5 | 1 | UniProt (Q14181) Pfam (PF08418) Pfam (PF22062) Pfam (PF04042) | Homo sapiens (human) | DNA polymerase alpha 70 kDa subunit |
| 5 | E (E) | CST complex subunit CTC1 | polymer | 1221 | 135050.3 | 1 | UniProt (Q2NKJ3) Pfam (PF15489) | Homo sapiens (human) | Conserved telomere maintenance component 1,HBV DNAPTP1-transactivated protein B |
| 6 | F (F) | CST complex subunit STN1 | polymer | 368 | 42172.9 | 1 | UniProt (Q9H668) Pfam (PF01336) Pfam (PF09170) | Homo sapiens (human) | Oligonucleotide/oligosaccharide-binding fold-containing protein 1,Suppressor of cdc thirteen homolog |
| 7 | G (G) | CST complex subunit TEN1 | polymer | 123 | 13872.0 | 1 | UniProt (Q86WV5) Pfam (PF15490) | Homo sapiens (human) | Protein telomeric pathways with STN1 homolog,Telomere length regulation protein TEN1 homolog |
| 8 | H (H) | canonical telomeric DNA sequence | polymer | 18 | 5682.7 | 1 | Homo sapiens | ||
| 9 | I, K, L, M (A, C, E) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 10 | J (B) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) |
Sequence modifications
B: 1 - 509 (UniProt: P49643)
C: 335 - 1462 (UniProt: P09884)
E: 1 - 1217 (UniProt: Q2NKJ3)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 331 | - | expression tag |
| Pro 332 | - | expression tag |
| Gly 333 | - | expression tag |
| Ser 334 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 501501.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 613.3 | |
| All* | Total formula weight | 502115.0 |
