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- EMDB-26346: Cryo-EM structure of human CST bound to DNA polymerase alpha-prim... -
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Open data
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Basic information
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Title | Cryo-EM structure of human CST bound to DNA polymerase alpha-primase in a recruitment state | |||||||||
![]() | Human CST-Pol alpha/Primase bound to ssDNA in a recruitment state. | |||||||||
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![]() | Fill-in / ![]() ![]() ![]() | |||||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Cai SW / Zinder JC / Svetlov V / Bush MW / Nudler E / Walz T / de Lange T | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of the human CST-Polα/primase complex in a recruitment state. Authors: Sarah W Cai / John C Zinder / Vladimir Svetlov / Martin W Bush / Evgeny Nudler / Thomas Walz / Titia de Lange / ![]() Abstract: The CST-Polα/primase complex is essential for telomere maintenance and functions to counteract resection at double-strand breaks. We report a 4.6-Å resolution cryo-EM structure of human CST- ...The CST-Polα/primase complex is essential for telomere maintenance and functions to counteract resection at double-strand breaks. We report a 4.6-Å resolution cryo-EM structure of human CST-Polα/primase, captured prior to catalysis in a recruitment state stabilized by chemical cross-linking. Our structure reveals an evolutionarily conserved interaction between the C-terminal domain of the catalytic POLA1 subunit and an N-terminal expansion in metazoan CTC1. Cross-linking mass spectrometry and negative-stain EM analysis provide insight into CST binding by the flexible POLA1 N-terminus. Finally, Coats plus syndrome disease mutations previously characterized to disrupt formation of the CST-Polα/primase complex map to protein-protein interfaces observed in the recruitment state. Together, our results shed light on the architecture and stoichiometry of the metazoan fill-in machinery. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 95.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 27.8 KB 27.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.7 KB | Display | ![]() |
Images | ![]() | 96.1 KB | ||
Filedesc metadata | ![]() | 9.1 KB | ||
Others | ![]() ![]() | 80.9 MB 80.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7u5cMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
EM raw data | ![]() Data size: 869.0 Data #1: Motion corrected micrographs of CST-Pola/Primase deltaN complex in recruitment state [micrographs - single frame]) |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Human CST-Pol alpha/Primase bound to ssDNA in a recruitment state. | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.08 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_26346_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_26346_half_map_2.map | ||||||||||||
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Density Histograms |
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Sample components
+Entire : Human CST bound to DNA polymerase alpha(POLA1 delta N)-primase in...
+Supramolecule #1: Human CST bound to DNA polymerase alpha(POLA1 delta N)-primase in...
+Macromolecule #1: DNA primase small subunit
+Macromolecule #2: DNA primase large subunit
+Macromolecule #3: DNA polymerase alpha catalytic subunit
+Macromolecule #4: DNA polymerase alpha subunit B
+Macromolecule #5: CST complex subunit CTC1
+Macromolecule #6: CST complex subunit STN1
+Macromolecule #7: CST complex subunit TEN1
+Macromolecule #8: canonical telomeric DNA sequence
+Macromolecule #9: ZINC ION
+Macromolecule #10: IRON/SULFUR CLUSTER
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Concentration | 0.075 mg/mL |
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Buffer | pH: 7.5 |
Grid | Model: Quantifoil R1.2/1.3 / Material: GOLD / Support film - Material: GRAPHENE OXIDE / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 278 K / Instrument: FEI VITROBOT MARK IV |
Details | The sample was cross-linked with glutaraldehyde (GraFix) |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 52.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |