7TX6
Cryo-EM structure of the human reduced folate carrier in complex with methotrexate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Reduced folate transporter,Soluble cytochrome b562 | polymer | 678 | 74797.2 | 1 | UniProt (P41440) UniProt (P0ABE7) Pfam (PF01770) Pfam (PF07361) In PDB | Homo sapiens (human) | FOLT,Intestinal folate carrier 1,IFC-1,Placental folate transporter,Reduced folate carrier protein,RFC,hRFC,Reduced folate transporter 1,RFT-1,Solute carrier family 19 member 1,hSLC19A1,Cytochrome b-562 |
2 | A | METHOTREXATE | non-polymer | 454.4 | 1 | Chemie (MTX) | FOLT,Intestinal folate carrier 1,IFC-1,Placental folate transporter,Reduced folate carrier protein,RFC,hRFC,Reduced folate transporter 1,RFT-1,Solute carrier family 19 member 1,hSLC19A1,Cytochrome b-562 | ||
3 | A | Digitonin | non-polymer | 1229.3 | 1 | Chemie (AJP) |
Sequence modifications
A: 1 - 211 (UniProt: P41440)
A: 211 - 215 (UniProt: P0ABE7)
A: 215 - 591 (UniProt: P41440)
A: 211 - 215 (UniProt: P0ABE7)
PDB | External Database | Details |
---|---|---|
Trp 211 | Met 29 | engineered mutation |
Ile 214 | His 124 | engineered mutation |
Leu 215 | - | linker |
PDB | External Database | Details |
---|---|---|
Ala 592 | - | expression tag |
Asn 593 | - | expression tag |
Ser 594 | - | expression tag |
Leu 595 | - | expression tag |
Glu 596 | - | expression tag |
Val 597 | - | expression tag |
Leu 598 | - | expression tag |
Phe 599 | - | expression tag |
Gln 600 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 74797.2 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 1683.8 | |
All* | Total formula weight | 76480.9 |