7TX6

Cryo-EM structure of the human reduced folate carrier in complex with methotrexate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Reduced folate transporter,Soluble cytochrome b562	polymer	678	74797.2	1	UniProt (P41440) UniProt (P0ABE7) Pfam (PF01770) Pfam (PF07361)	Homo sapiens (human)	FOLT,Intestinal folate carrier 1,IFC-1,Placental folate transporter,Reduced folate carrier protein,RFC,hRFC,Reduced folate transporter 1,RFT-1,Solute carrier family 19 member 1,hSLC19A1,Cytochrome b-562
2	B (A)	METHOTREXATE	non-polymer		454.4	1	Chemie (MTX)		FOLT,Intestinal folate carrier 1,IFC-1,Placental folate transporter,Reduced folate carrier protein,RFC,hRFC,Reduced folate transporter 1,RFT-1,Solute carrier family 19 member 1,hSLC19A1,Cytochrome b-562
3	C (A)	Digitonin	non-polymer		1229.3	1	Chemie (AJP)

Sequence modifications

A: 1 - 211 (UniProt: P41440)
A: 211 - 215 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 211	Met 29	engineered mutation
Ile 214	His 124	engineered mutation
Leu 215	-	linker

A: 215 - 591 (UniProt: P41440)

PDB	External Database	Details
Ala 592	-	expression tag
Asn 593	-	expression tag
Ser 594	-	expression tag
Leu 595	-	expression tag
Glu 596	-	expression tag
Val 597	-	expression tag
Leu 598	-	expression tag
Phe 599	-	expression tag
Gln 600	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	74797.2
Non-Polymers*	Number of molecules	2
	Total formula weight	1683.8
All*	Total formula weight	76480.9

*Water molecules are not included.