7TUZ
Cryo-EM structure of 7alpha,25-dihydroxycholesterol-bound EBI2/GPR183 in complex with Gi protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | G-protein coupled receptor 183 | polymer | 384 | 43840.5 | 1 | UniProt (P32249) Pfam (PF00001) | Homo sapiens (human) | Epstein-Barr virus-induced G-protein coupled receptor 2,EBI2,EBV-induced G-protein coupled receptor 2,hEBI2 |
| 2 | B (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40398.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 356 | 39141.8 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (E) | scFv16 | polymer | 266 | 28636.8 | 1 | Mus musculus (Mouse) | ||
| 6 | F (R) | (2S,4aS,4bS,7R,8S,8aS,9R,10aR)-7-[(2R,3R)-7-hydroxy-3,7-dimethyloctan-2-yl]-4a,7,8-trimethyltetradecahydrophenanthrene-2,9-diol | non-polymer | 418.7 | 1 | Chemie (KJX) PubChem (24779596) PubChem (42622606) PubChem (473142) PubChem (53695891) PubChem (53695892) PubChem (53695893) PubChem (124893089) PubChem (154997478) PubChem (58455548) PubChem (58455552) PubChem (68219058) PubChem (89939081) PubChem (129922) PubChem (126969882) PubChem (10251477) PubChem (11774434) PubChem (11954197) PubChem (14077700) PubChem (132053022) PubChem (136212458) PubChem (146159411) PubChem (157603244) PubChem (158934800) PubChem (168841982) PubChem (169755115) PubChem (172432054) |
Sequence modifications
R: 2 - 361 (UniProt: P32249)
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Val 0 | - | expression tag |
| Asp 1 | - | expression tag |
| Ala 362 | - | expression tag |
| Ala 363 | - | expression tag |
| Ala 364 | - | expression tag |
| His 365 | - | expression tag |
| His 366 | - | expression tag |
| His 367 | - | expression tag |
| His 368 | - | expression tag |
| His 369 | - | expression tag |
| His 370 | - | expression tag |
| His 371 | - | expression tag |
| His 372 | - | expression tag |
| Gly 373 | - | expression tag |
| Gly 374 | - | expression tag |
| Asp 375 | - | expression tag |
| Tyr 376 | - | expression tag |
| Lys 377 | - | expression tag |
| Asp 378 | - | expression tag |
| Asp 379 | - | expression tag |
| Asp 380 | - | expression tag |
| Asp 381 | - | expression tag |
| Lys 382 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | conflict |
| Ala 203 | Gly 203 | conflict |
| Ala 245 | Glu 245 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 159878.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 418.7 | |
| All* | Total formula weight | 160296.9 |
