7TUZ
Cryo-EM structure of 7alpha,25-dihydroxycholesterol-bound EBI2/GPR183 in complex with Gi protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | G-protein coupled receptor 183 | polymer | 384 | 43840.5 | 1 | UniProt (P32249) Pfam (PF00001) | Homo sapiens (human) | Epstein-Barr virus-induced G-protein coupled receptor 2,EBI2,EBV-induced G-protein coupled receptor 2,hEBI2 |
| 2 | B (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40398.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 356 | 39141.8 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (E) | scFv16 | polymer | 266 | 28636.8 | 1 | Mus musculus (Mouse) | ||
| 6 | F (R) | (2S,4aS,4bS,7R,8S,8aS,9R,10aR)-7-[(2R,3R)-7-hydroxy-3,7-dimethyloctan-2-yl]-4a,7,8-trimethyltetradecahydrophenanthrene-2,9-diol | non-polymer | 418.7 | 1 | Chemie (KJX) |
Sequence modifications
R: 2 - 361 (UniProt: P32249)
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Val 0 | - | expression tag |
| Asp 1 | - | expression tag |
| Ala 362 | - | expression tag |
| Ala 363 | - | expression tag |
| Ala 364 | - | expression tag |
| His 365 | - | expression tag |
| His 366 | - | expression tag |
| His 367 | - | expression tag |
| His 368 | - | expression tag |
| His 369 | - | expression tag |
| His 370 | - | expression tag |
| His 371 | - | expression tag |
| His 372 | - | expression tag |
| Gly 373 | - | expression tag |
| Gly 374 | - | expression tag |
| Asp 375 | - | expression tag |
| Tyr 376 | - | expression tag |
| Lys 377 | - | expression tag |
| Asp 378 | - | expression tag |
| Asp 379 | - | expression tag |
| Asp 380 | - | expression tag |
| Asp 381 | - | expression tag |
| Lys 382 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | conflict |
| Ala 203 | Gly 203 | conflict |
| Ala 245 | Glu 245 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 159878.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 418.7 | |
| All* | Total formula weight | 160296.9 |
