7TRY

Cryo-EM structure of corticotropin releasing factor receptor 2 bound to Urocortin 1 and coupled with heterotrimeric G11 protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (P)	Corticotropin-releasing factor receptor 2	polymer	560	63716.2	1	UniProt (Q13324) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (human)	CRF-R-2,CRF-R2,CRFR-2,Corticotropin-releasing hormone receptor 2,CRH-R-2,CRH-R2
2	B (U)	Urocortin	polymer	40	4703.3	1	UniProt (P55089) Pfam (PF00473)	Homo sapiens
3	C (A)	Guanine nucleotide-binding protein subunit alpha-11	polymer	353	41415.3	1	UniProt (P29992) Pfam (PF00503)	Homo sapiens (human)	G alpha-11,G-protein subunit alpha-11,Guanine nucleotide-binding protein G(y) subunit alpha
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	400	43706.8	1	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
5	E (G)	G protein gamma subunit	polymer	71	7861.1	1	Pfam (PF00631) UniProt (by SIFTS) (A0A9X9ZA26)	Rattus norvegicus (Norway rat)
6	F (H)	scFV16	polymer	247	26277.3	1		synthetic construct

Sequence modifications

P: 2 - 388 (UniProt: Q13324)
P: 389 - 561 (PDB: 7TRY)
A: 25 - 359 (UniProt: P29992)

PDB	External Database	Details
Met 7	-	expression tag
Gly 8	-	expression tag
Cys 9	-	expression tag
Thr 10	-	expression tag
Leu 11	-	expression tag
Ser 12	-	expression tag
Ala 13	-	expression tag
Glu 14	-	expression tag
Asp 15	-	expression tag
Lys 16	-	expression tag
Ala 17	-	expression tag
Ala 18	-	expression tag
Val 19	-	expression tag
Glu 20	-	expression tag
Arg 21	-	expression tag
Ser 22	-	expression tag
Lys 23	-	expression tag
Met 24	-	expression tag
Ala 208	Gly 208	conflict
Ser 331	Ala 331	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -33	-	expression tag
His -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
Ser -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Leu -23	-	expression tag
Val -22	-	expression tag
Pro -21	-	expression tag
Arg -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Met -16	-	expression tag
Ala -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	187680.1
All*	Total formula weight	187680.1

*Water molecules are not included.