7TMW

Cryo-EM structure of the relaxin receptor RXFP1 in complex with heterotrimeric Gs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Relaxin receptor 1, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short fusion	polymer	984	113442.9	1	UniProt (Q9HBX9) UniProt (P63092) Pfam (PF00057) Pfam (PF13855) Pfam (PF00001) Pfam (PF00503)	Homo sapiens (human)	Leucine-rich repeat-containing G-protein coupled receptor 7,Relaxin family peptide receptor 1, Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Camelid antibody VHH fragment Nb35	polymer	159	17444.3	1		Lama glama (llama)

Sequence modifications

R: 23 - 1000 (UniProt: Q9HBX9)

PDB	External Database	Details
Asp 2	-	expression tag
Tyr 3	-	expression tag
Lys 4	-	expression tag
Asp 5	-	expression tag
Asp 6	-	expression tag
Asp 7	-	expression tag
Asp 8	-	expression tag
Gly 9	-	expression tag
Gly 10	-	expression tag
Ser 11	-	expression tag
Leu 12	-	expression tag
Glu 13	-	expression tag
Val 14	-	expression tag
Leu 15	-	expression tag
Phe 16	-	expression tag
Gln 17	-	expression tag
Gly 18	-	expression tag
Pro 19	-	expression tag
Gly 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
Asn 1001	-	linker
Ser 1002	-	linker
Lys 1003	-	linker
Thr 1004	-	linker
Glu 1005	-	linker
Asp 1006	-	linker
Gln 1007	-	linker
Arg 1008	-	linker
Asn 1009	-	linker
Glu 1010	-	linker
Glu 1011	-	linker
Lys 1012	-	linker
Ala 1013	-	linker
Gln 1014	-	linker
Arg 1015	-	linker
Glu 1016	-	linker
Ala 1017	-	linker
Asn 1018	-	linker
Lys 1019	-	linker
Lys 1020	-	linker
Ile 1021	-	linker
Glu 1022	-	linker
Lys 1023	-	linker
Gln 1024	-	linker
Leu 1025	-	linker
Gln 1026	-	linker
Lys 1027	-	linker
Asp 1028	-	linker
Lys 1029	-	linker
Gln 1030	-	linker
Val 1031	-	linker
Tyr 1032	-	linker
Arg 1033	-	linker
Ala 1034	-	linker
Thr 1035	-	linker
His 1036	-	linker
Arg 1037	-	linker
Leu 1038	-	linker
Leu 1039	-	linker
Leu 1040	-	linker
Leu 1041	-	linker
Gly 1042	-	linker
Ala 1043	-	linker
Asp 1044	-	linker
Asn 1045	-	linker
Ser 1046	-	linker
Gly 1047	-	linker
Lys 1048	-	linker
Ser 1049	-	linker
Thr 1050	-	linker
Ile 1051	-	linker
Val 1052	-	linker
Lys 1053	-	linker
Gln 1054	-	linker
Met 1055	-	linker
Arg 1056	-	linker
Ile 1057	-	linker
Tyr 1058	-	linker
His 1190	-	linker
Gly 1191	-	linker
Gly 1192	-	linker
Ser 1193	-	linker
Gly 1194	-	linker
Gly 1195	-	linker
Ser 1196	-	linker
Gly 1197	-	linker
Gly 1198	-	linker

R: 1199 - 1379 (UniProt: P63092)

PDB	External Database	Details
Asp 1244	Ala 249	conflict
Asp 1247	Ser 252	conflict
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 1357	Ile 372	conflict
Ile 1360	Val 375	conflict

B: 12 - 350 (UniProt: P62873)

PDB	External Database	Details
Met 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Ser 10	-	expression tag
Gly 11	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	177282.4
All*	Total formula weight	177282.4

*Water molecules are not included.