7TGH

Cryo-EM structure of respiratory super-complex CI+III2 from Tetrahymena thermophila

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	1	NADH-ubiquinone oxidoreductase chain 1	polymer	284	32636.8	1	UniProt (Q950Y3) Pfam (PF00146) In PDB	Tetrahymena thermophila
2	1B	NADH dehydrogenase subunit 1	polymer	59	7238.8	1	UniProt (Q09FB0) Pfam (PF00146) In PDB	Tetrahymena thermophila
3	2	Ymf65	polymer	360	43104.1	1	UniProt (Q951A3) Pfam (PF00361) In PDB	Tetrahymena thermophila
4	2B	NADH dehydrogenase subunit 2	polymer	178	20899.9	1	UniProt (Q951B2) Pfam (PF00361) In PDB	Tetrahymena thermophila
5	3	NADH-ubiquinone oxidoreductase chain 3	polymer	121	14416.9	1	UniProt (Q950Z7) Pfam (PF00507) In PDB	Tetrahymena thermophila
6	3A, 3a	M16 family peptidase, putative	polymer	482	53030.5	2	UniProt (I7MJ25) Pfam (PF05193) In PDB	Tetrahymena thermophila
7	3B, 3b	Peptidase M16 inactive domain protein	polymer	513	58023.9	2	UniProt (I7MGU2) Pfam (PF00675) Pfam (PF05193) In PDB	Tetrahymena thermophila
8	3C, 3c	Apocytochrome b	polymer	426	50635.5	2	UniProt (Q950Z1) Pfam (PF00033) In PDB	Tetrahymena thermophila
9	3D, 3d	Cytochrome protein c1	polymer	319	37616.6	2	UniProt (Q24IM5) Pfam (PF02167) In PDB	Tetrahymena thermophila
10	3E, 3e	Rieske iron-sulfur protein, ubiquinol-cytochrome C reductase iron-sulfur subunit	polymer	269	30697.0	2	UniProt (I7MIC7) Pfam (PF00355) In PDB	Tetrahymena thermophila
11	3F, 3f	Ubiquinol-cytochrome C reductase hinge protein	polymer	90	10250.0	2	UniProt (Q22AX2) Pfam (PF02320) In PDB	Tetrahymena thermophila
12	3G, 3g	UQCRB	polymer	328	39193.5	2	UniProt (Q23F81) In PDB	Tetrahymena thermophila
13	3H, 3h	Transmembrane protein, putative	polymer	130	15677.8	2	UniProt (I7M484) In PDB	Tetrahymena thermophila
14	3I, 3i	Transmembrane protein, putative	polymer	119	14262.6	2	UniProt (I7MM45) In PDB	Tetrahymena thermophila
15	3J, 3j	Transmembrane protein, putative	polymer	62	7455.7	2	UniProt (I7MFL6) In PDB	Tetrahymena thermophila
16	3M	UNK1	polymer	19	2181.6	1		Tetrahymena thermophila
17	3l	UNK2	polymer	24	1781.9	1		Tetrahymena thermophila
18	3m	UNK3	polymer	17	1725.0	1		Tetrahymena thermophila
19	4	NADH-ubiquinone oxidoreductase chain 4	polymer	505	58690.0	1	UniProt (Q950X9) Pfam (PF00361) In PDB	Tetrahymena thermophila
20	4L	Ymf58	polymer	116	13509.9	1	UniProt (Q950Z5) In PDB	Tetrahymena thermophila
21	5	NADH dehydrogenase subunit 5	polymer	750	88179.8	1	UniProt (Q950Z0) Pfam (PF00361) In PDB	Tetrahymena thermophila
22	5B	Ymf57	polymer	100	12473.9	1	UniProt (Q951C2) In PDB	Tetrahymena thermophila
23	6	Ymf62	polymer	255	30448.5	1	UniProt (Q950Y2) In PDB	Tetrahymena thermophila
24	A2	Ribosomal protein L51/S25/CI-B8 domain protein	polymer	103	12157.8	1	UniProt (I7MA77) Pfam (PF05047) In PDB	Tetrahymena thermophila
25	A5	ETC complex I subunit motif protein	polymer	206	24277.9	1	UniProt (Q23ND5) Pfam (PF04716) In PDB	Tetrahymena thermophila
26	A6	NADH dehydrogenase, putative	polymer	172	20167.9	1	UniProt (I7M2Y3) In PDB	Tetrahymena thermophila
27	A7	NDUA7	polymer	282	33201.8	1	UniProt (I7MIJ7) In PDB	Tetrahymena thermophila
28	A9	NAD-dependent epimerase/dehydratase family protein	polymer	362	41375.1	1	UniProt (I7MLH2) Pfam (PF01370) In PDB	Tetrahymena thermophila
29	AB	Acyl carrier protein	polymer	138	16249.4	1	UniProt (Q22XT6) Pfam (PF00550) In PDB	Tetrahymena thermophila
30	AC	Acyl carrier protein	polymer	133	15428.4	1	UniProt (I7MD12) Pfam (PF00550) In PDB	Tetrahymena thermophila
31	AL	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12	polymer	194	22890.6	1	UniProt (A4VDQ6) Pfam (PF05071) In PDB	Tetrahymena thermophila
32	AM	NDUA13	polymer	175	21045.6	1	UniProt (I7M2U4) In PDB	Tetrahymena thermophila
33	B7	NDUB7	polymer	120	13747.4	1	UniProt (I7MIM0) In PDB	Tetrahymena thermophila
34	B8	NDUB8	polymer	207	24150.5	1	UniProt (I7M855) In PDB	Tetrahymena thermophila
35	BL	NDUB10	polymer	188	22167.1	1	UniProt (Q23KG0) In PDB	Tetrahymena thermophila
36	C	UNK4	polymer	209	17804.9	1		Tetrahymena thermophila
37	C1	Gamma-carbonic anhydrase	polymer	257	28216.7	1	UniProt (Q22XU5) Pfam (PF21711) In PDB	Tetrahymena thermophila
38	C2	Gamma-carbonic anhydrase	polymer	233	25396.0	1	UniProt (I7M6S0) Pfam (PF21711) In PDB	Tetrahymena thermophila
39	C3	Transcription factor apfi protein, putative	polymer	346	39793.4	1	UniProt (I7M8Q7) Pfam (PF00132) In PDB	Tetrahymena thermophila
40	TD	Transmembrane protein, putative	polymer	73	8787.1	1	UniProt (Q22DC2) In PDB	Tetrahymena thermophila
41	J1	DnaJ domain protein	polymer	317	36878.4	1	UniProt (Q22DR7) Pfam (PF00226) In PDB	Tetrahymena thermophila
42	FX	2 iron, 2 sulfur cluster-binding protein	polymer	172	19564.2	1	UniProt (Q22W11) Pfam (PF00111) In PDB	Tetrahymena thermophila
43	T2	Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II	polymer	333	36709.4	1	UniProt (Q24HK5) Pfam (PF00501) In PDB	Tetrahymena thermophila
44	T1	Lipid-A-disaccharide synthase	polymer	516	59307.3	1	UniProt (Q22E24) Pfam (PF02684) In PDB	Tetrahymena thermophila
45	A1	NDUA1	polymer	94	11451.2	1	UniProt (I7MI60) In PDB	Tetrahymena thermophila
46	X1	NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit	polymer	150	17381.8	1	UniProt (I7LT42) Pfam (PF10785) In PDB	Tetrahymena thermophila
47	T6	NDUTT6	polymer	144	17078.3	1	UniProt (I7M1N8) In PDB	Tetrahymena thermophila
48	T5	Transmembrane protein, putative	polymer	205	23971.0	1	UniProt (I7LT77) In PDB	Tetrahymena thermophila
49	R	UNK5	polymer	124	13356.4	1		Tetrahymena thermophila
50	S1	NADH-ubiquinone oxidoreductase 75 kDa subunit	polymer	718	80580.8	1	UniProt (Q23KA9) Pfam (PF13510) Pfam (PF10588) Pfam (PF00384) Pfam (PF09326) In PDB	Tetrahymena thermophila
51	S2	NADH dehydrogenase subunit 7	polymer	442	51227.5	1	UniProt (Q951B1) Pfam (PF00346) In PDB	Tetrahymena thermophila
52	S3	NADH dehydrogenase subunit 9	polymer	198	23803.3	1	UniProt (Q950Z4) Pfam (PF00329) In PDB	Tetrahymena thermophila
53	S4	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial	polymer	185	21642.4	1	UniProt (I7MK61) Pfam (PF04800) In PDB	Tetrahymena thermophila
54	S6	Zinc-finger protein	polymer	132	15141.3	1	UniProt (I7MK02) Pfam (PF10276) In PDB	Tetrahymena thermophila
55	S7	NADH dehydrogenase subunit 10	polymer	162	18324.3	1	UniProt (Q951B4) Pfam (PF01058) In PDB	Tetrahymena thermophila
56	S8	NADH-ubiquinone oxidoreductase 1, chain, putative	polymer	236	28053.5	1	UniProt (I7MDW5) Pfam (PF12838) In PDB	Tetrahymena thermophila
57	B9	NDUB9	polymer	189	22698.3	1	UniProt (Q233X7) In PDB	Tetrahymena thermophila
58	TX	Thioredoxin	polymer	166	19540.2	1	UniProt (Q22AI5) Pfam (PF00085) In PDB	Tetrahymena thermophila
59	A8	NDUA8	polymer	238	28412.8	1	UniProt (I7MMF4) In PDB	Tetrahymena thermophila
60	TB	Transmembrane protein, putative	polymer	113	13341.0	1	UniProt (Q22T55) In PDB	Tetrahymena thermophila
61	V1	NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial	polymer	474	52313.7	1	UniProt (Q23KE4) Pfam (PF01512) Pfam (PF10531) Pfam (PF10589) In PDB	Tetrahymena thermophila
62	V2	NADH-ubiquinone oxidoreductase 24 kDa subunit	polymer	274	31216.7	1	UniProt (I7MEP0) Pfam (PF01257) In PDB	Tetrahymena thermophila
63	B6	NDUB6	polymer	129	15444.3	1	UniProt (Q231G0) In PDB	Tetrahymena thermophila
64	BM	Transmembrane protein, putative	polymer	214	24419.2	1	UniProt (Q22Z32) In PDB	Tetrahymena thermophila
65	C4	NDUC2	polymer	102	12103.0	1	UniProt (Q22W63) In PDB	Tetrahymena thermophila
66	AN	Transmembrane protein, putative	polymer	231	26545.1	1	UniProt (Q24F24) In PDB	Tetrahymena thermophila
67	B4	NDUB4	polymer	108	12578.0	1		Tetrahymena thermophila
68	T4	NDUTT4	polymer	212	24823.3	1	UniProt (I7MIE0) In PDB	Tetrahymena thermophila
69	T8	NDUTT8	polymer	135	15785.2	1	UniProt (Q22SC4) In PDB	Tetrahymena thermophila
70	B2	NDUB2	polymer	126	14410.4	1	UniProt (I7MG29) In PDB	Tetrahymena thermophila
71	T3	NDUTT3	polymer	311	35409.2	1	UniProt (I7LUQ4) In PDB	Tetrahymena thermophila
72	P1	Transmembrane protein, putative	polymer	251	29389.7	1	UniProt (Q24C39) In PDB	Tetrahymena thermophila
73	B3	Transmembrane protein, putative	polymer	83	9942.4	1	UniProt (A4VD20) In PDB	Tetrahymena thermophila
74	TA	NDUTT10	polymer	102	12123.9	1	UniProt (I7MAF0) In PDB	Tetrahymena thermophila
75	S5	GRAM domain protein	polymer	94	10920.4	1	UniProt (W7X4R4) In PDB	Tetrahymena thermophila
76	TC	NDUTT12	polymer	93	11283.5	1	UniProt (Q22E95) In PDB	Tetrahymena thermophila
77	P2	NDUPH2	polymer	189	22446.6	1	UniProt (Q23KE0) In PDB	Tetrahymena thermophila
78	A3	Transmembrane protein, putative	polymer	135	15831.7	1	UniProt (I7M9B3) In PDB	Tetrahymena thermophila
79	T9	NDUTT9	polymer	135	15576.6	1	UniProt (Q23B10) In PDB	Tetrahymena thermophila
80	TE	Transmembrane protein, putative	polymer	71	8445.9	1	UniProt (I7MIK1) In PDB	Tetrahymena thermophila
81	T7	Transmembrane protein, putative	polymer	143	16909.2	1	UniProt (Q22HE4) In PDB	Tetrahymena thermophila
82	TD, AN, 3i, B4, T1...	CARDIOLIPIN	non-polymer		1464.0	29	Chemie (CDL)
83	3e, AN, 3g, J1, 3E...	1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		790.1	32	Chemie (PC1)
84	3C, 3c	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)
85	3H, 5B, 3C	UBIQUINONE-10	non-polymer		863.3	3	Chemie (U10)
86	3D, 3d	HEME C	non-polymer		618.5	2	Chemie (HEC)
87	3E, FX, S1, V2	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	4	Chemie (FES)
88	3E, T4, 4, T8, P1...	1,2-Distearoyl-sn-glycerophosphoethanolamine	non-polymer		748.1	17	Chemie (3PE)
89	3F, S6, A9	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
90	A9	NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		745.4	1	Chemie (NDP)
91	AB	S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate	non-polymer		568.7	1	Chemie (ZMP)
92	B8	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
93	S1, S7, S8, V1	IRON/SULFUR CLUSTER	non-polymer		351.6	6	Chemie (SF4)
94	V1	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)

Sequence modifications

1B: 1 - 59 (UniProt: Q09FB0)

PDB	External Database	Details
Val 49	Leu 49	conflict
Thr 56	Ser 56	conflict

Contents of the asymmetric unit

Polymers	Number of chains	91
	Total formula weight	2324212.6
Non-Polymers*	Number of molecules	104
	Total formula weight	91959.0
All*	Total formula weight	2416171.6

*Water molecules are not included.