7TFN
Cryo-EM structure of CD4bs antibody Ab1303 in complex with HIV-1 Env trimer BG505 SOSIP.664
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Envelope glycoprotein BG505 SOSIP.664 - gp120 | polymer | 481 | 54064.3 | 3 | UniProt (A0A6H1VH54) Pfam (PF00516) UniProt (by SIFTS) (Q2N0S6) | Human immunodeficiency virus 1 | |
| 2 | D, E, H (H, I, P) | Anti-HIV-1 CD4bs antibody Fab Ab1303 - Heavy chain | polymer | 235 | 25338.2 | 3 | Macaca mulatta (Rhesus monkey) | ||
| 3 | F, G, I (J, L, Q) | CD4 binding site antibody Fab Ab1303 - light chain | polymer | 217 | 23420.0 | 3 | Macaca mulatta (Rhesus monkey) | ||
| 4 | J, K, L (X, Y, Z) | Envelope glycoprotein BG505 SOSIP.664 - gp41 | polymer | 153 | 17146.5 | 3 | UniProt (Q2N0S6) Pfam (PF00517) | Human immunodeficiency virus 1 | Env polyprotein |
| 5 | M (D) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 6 | AA, BA, N, S (b, c, E, O) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 4 | In PDB GlyTouCan (G15407YE) | |||
| 7 | O, R, T, V, X... (F, N, R, T, V...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 6 | In PDB GlyTouCan (G42666HT) | |||
| 8 | P (G) | alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G22573RC) | |||
| 9 | Q, U, W, Y (M, S, U, W) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 4 | In PDB GlyTouCan (G81315DD) | |||
| 10 | CA, DA, EA, FA, GA... (A, B, C) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 8 | Chemie (NAG) | |||
| 11 | IA (C) | alpha-D-mannopyranose | non-polymer | 180.2 | 1 | Chemie (MAN) |
Sequence modifications
A, B, C: 31 - 511 (UniProt: A0A6H1VH54)
X, Y, Z: 512 - 664 (UniProt: Q2N0S6)
| PDB | External Database | Details |
|---|---|---|
| Lys 64 | Glu 63 | conflict |
| Ser 375 | Tyr 373 | conflict |
| Cys 501 | Ala 498 | conflict |
| Arg 509 | Glu 506 | conflict |
| Arg 512 | - | expression tag |
| Arg 513 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | conflict |
| Cys 605 | Thr 602 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 359906.9 | |
| Branched | Number of molecules | 16 |
| Total formula weight | 9546.8 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1949.8 | |
| All* | Total formula weight | 371403.6 |
