7T94
Cryo-EM structure of S1 state ACh-bound M2R-Go signaling complex with a PAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Muscarinic acetylcholine receptor M2,muscarinic acetylcholine receptor M2 chimera | polymer | 353 | 39729.9 | 1 | UniProt (P08172) Pfam (PF00001) | Homo sapiens (human) | |
| 2 | B (B) | Guanine nucleotide-binding protein G(o) subunit alpha | polymer | 354 | 40104.6 | 1 | UniProt (P09471) Pfam (PF00503) | Homo sapiens (human) | |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37728.2 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (D) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (E) | Antibody fragment | polymer | 256 | 27340.5 | 1 | Mus musculus | ||
| 6 | F (A) | ACETYLCHOLINE | non-polymer | 146.2 | 1 | Chemie (ACH) | |||
| 7 | G (A) | 3-amino-5-chloro-N-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide | non-polymer | 437.9 | 1 | Chemie (2CU) | |||
| 8 | H (A) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 4 - 359 (UniProt: P08172)
A: 368 - 466 (UniProt: P08172)
B: 1 - 354 (UniProt: P09471)
C: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asp -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Asp 1 | - | expression tag |
| Asp 2 | - | expression tag |
| Ala 3 | - | expression tag |
| Asp 6 | Asn 6 | conflict |
| Asp 9 | Asn 9 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ile 360 | - | linker |
| Val 361 | - | linker |
| Ala 362 | - | linker |
| Arg 363 | - | linker |
| Lys 364 | - | linker |
| Ile 365 | - | linker |
| Val 366 | - | linker |
| Lys 367 | - | linker |
| Pro 467 | - | expression tag |
| Ala 468 | - | expression tag |
| Gly 469 | - | expression tag |
| Leu 470 | - | expression tag |
| Glu 471 | - | expression tag |
| Val 472 | - | expression tag |
| Leu 473 | - | expression tag |
| Phe 474 | - | expression tag |
| Gln 475 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp 9 | Glu 9 | engineered mutation |
| Lys 10 | Arg 10 | engineered mutation |
| Val 13 | Leu 13 | engineered mutation |
| Met 18 | Ala 18 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 152764.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 584.2 | |
| All* | Total formula weight | 153348.4 |
