7T93

Crystal Structure of Fumarate hydratase class II from Mycobacterium ulcerans in complex with L-Malate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Fumarate hydratase class II	polymer	496	52471.2	4	UniProt (A0PKR5) Pfam (PF00206) Pfam (PF10415)	Mycobacterium ulcerans Agy99	Fumarase C,Aerobic fumarase,Iron-independent fumarase
2	E, F, H, I, J... (A, B, C, D)	(2S)-2-hydroxybutanedioic acid	non-polymer		134.1	6	Chemie (LMR)
3	G (B)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
4	L, M, N, O (A, B, C, D)	water	water		18.0	2165	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 475 (UniProt: A0PKR5)

PDB	External Database	Details
Met -20	-	initiating methionine
Ala -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Met -12	-	expression tag
Gly -11	-	expression tag
Thr -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Ala -7	-	expression tag
Gln -6	-	expression tag
Thr -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Thr 151	Ala 151	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	209884.7
Non-Polymers*	Number of molecules	7
	Total formula weight	922.7
All*	Total formula weight	210807.4

*Water molecules are not included.