7T3D

CryoEM map of anchor 222-1C06 Fab and lateral patch 2B05 Fab binding H1 HA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, G, M (A, E, F)	Hemagglutinin HA1 chain	polymer	331	36729.4	3	UniProt (C3W5S1) Pfam (PF00509)	Influenza A virus (A/California/04/2009(H1N1))
2	B, H, N (B, G, I)	Hemagglutinin HA2 chain	polymer	174	19977.1	3	UniProt (C3W5S1) Pfam (PF00509)	Influenza A virus (A/California/04/2009(H1N1))
3	C, I, O (D, M, N)	2B05 mAb light chain	polymer	107	11769.1	3		Homo sapiens
4	D, J, P (C, J, K)	2B05 mAb heavy chain	polymer	120	12805.2	3		Homo sapiens
5	E, K, Q (H, O, P)	222-1C06 mAb heavy chain	polymer	122	13528.0	3		Homo sapiens
6	F, L, R (L, Q, R)	222-1C06 mAb light chain	polymer	108	11657.0	3		Homo sapiens
7	S, T, U (S, T, U)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
8	AA, BA, CA, DA, EA... (E, G, F, I, A...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	15	Chemie (NAG)

Sequence modifications

A, E, F: 11 - 329 (UniProt: C3W5S1)

PDB	External Database	Details
Ala 7	-	expression tag
Asp 8	-	expression tag
Pro 9	-	expression tag
Gly 10	-	expression tag

B, G, I: 1 - 174 (UniProt: C3W5S1)

PDB	External Database	Details
Lys 47	Glu 391	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	319397.7
Branched	Number of molecules	3
	Total formula weight	1273.2
Non-Polymers*	Number of molecules	15
	Total formula weight	3318.1
All*	Total formula weight	323989.0

*Water molecules are not included.