7T3B

GATOR1-RAG-RAGULATOR - GAP Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E	Ras-related GTP-binding protein C	polymer	399	44195.7	1	UniProt (Q9HB90) Pfam (PF04670) In PDB	Homo sapiens (human)	Rag C,RagC,GTPase-interacting protein 2,TIB929
2	B	GATOR complex protein NPRL2	polymer	380	43711.4	1	UniProt (Q8WTW4) Pfam (PF06218) In PDB	Homo sapiens (human)	Gene 21 protein,G21 protein,Nitrogen permease regulator 2-like protein,NPR2-like protein,Tumor suppressor candidate 4
3	A	GATOR complex protein DEPDC5	polymer	1603	181385.9	1	UniProt (O75140) Pfam (PF12257) Pfam (PF00610) Pfam (PF19418) In PDB	Homo sapiens (human)	DEP domain-containing protein 5
4	H	Ragulator complex protein LAMTOR3	polymer	124	13637.7	1	UniProt (Q9UHA4) Pfam (PF08923) In PDB	Homo sapiens (human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3,MEK-binding partner 1,Mp1,Mitogen-activated protein kinase kinase 1-interacting protein 1,Mitogen-activated protein kinase scaffold protein 1
5	G	Ragulator complex protein LAMTOR2	polymer	125	13517.5	1	UniProt (Q9Y2Q5) Pfam (PF03259) In PDB	Homo sapiens (human)	Endosomal adaptor protein p14,Late endosomal/lysosomal Mp1-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2,Mitogen-activated protein-binding protein-interacting protein,MAPBP-interacting protein,Roadblock domain-containing protein 3
6	J	Ragulator complex protein LAMTOR5	polymer	91	9622.9	1	UniProt (O43504) Pfam (PF16672) In PDB	Homo sapiens (human)	Hepatitis B virus X-interacting protein,HBX-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5
7	I	Ragulator complex protein LAMTOR4	polymer	99	10753.2	1	UniProt (Q0VGL1) In PDB	Homo sapiens (human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4
8	F	Ragulator complex protein LAMTOR1	polymer	161	17762.8	1	UniProt (Q6IAA8) Pfam (PF15454) In PDB	Homo sapiens (human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1,Lipid raft adaptor protein p18,Protein associated with DRMs and endosomes,p27Kip1-releasing factor from RhoA,p27RF-Rho
9	C	GATOR complex protein NPRL3	polymer	569	63680.8	1	UniProt (Q12980) Pfam (PF03666) In PDB	Homo sapiens (human)	-14 gene protein,Alpha-globin regulatory element-containing gene protein,Nitrogen permease regulator 3-like protein,Protein CGTHBA
10	D	Ras-related GTP-binding protein A	polymer	313	36615.2	1	UniProt (Q7L523) Pfam (PF04670) In PDB	Homo sapiens (human)	RagA,Adenovirus E3 14.7 kDa-interacting protein 1,FIP-1
11	E, B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
12	E, D	ALUMINUM FLUORIDE	non-polymer		84.0	2	Chemie (AF3)

Sequence modifications

E: 1 - 399 (UniProt: Q9HB90)

PDB	External Database	Details
Ala 92	Phe 92	engineered mutation

A: 1 - 1603 (UniProt: O75140)

PDB	External Database	Details
Ala 775	Tyr 775	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	434883.1
Non-Polymers*	Number of molecules	4
	Total formula weight	1054.4
All*	Total formula weight	435937.4

*Water molecules are not included.