7T2G
CryoEM structure of mu-opioid receptor - Gi protein complex bound to mitragynine pseudoindoxyl (MP)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40415.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 344 | 37671.1 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (R) | Soluble cytochrome b562,Mu-type opioid receptor | polymer | 488 | 55123.8 | 1 | UniProt (P0ABE7) UniProt (P42866) Pfam (PF07361) Pfam (PF00001) | Escherichia coli | Cytochrome b-562,M-OR-1,MOR-1 |
| 5 | E (R) | Mitragynine pseudoindoxyl | non-polymer | 414.5 | 1 | Chemie (EIG) | |||
| 6 | F, G (R) | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
| 7 | H (R) | water | water | 18.0 | 8 | Chemie (HOH) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: -52 - 52 (UniProt: P0ABE7)
R: 73 - 406 (UniProt: P42866)
| PDB | External Database | Details |
|---|---|---|
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Cys -73 | - | expression tag |
| Leu -72 | - | expression tag |
| Val -71 | - | expression tag |
| Phe -70 | - | expression tag |
| Ala -69 | - | expression tag |
| Asp -68 | - | expression tag |
| Tyr -67 | - | expression tag |
| Lys -66 | - | expression tag |
| Asp -65 | - | expression tag |
| Asp -64 | - | expression tag |
| Asp -63 | - | expression tag |
| Asp -62 | - | expression tag |
| Ala -61 | - | expression tag |
| Leu -60 | - | expression tag |
| Glu -59 | - | expression tag |
| Val -58 | - | expression tag |
| Leu -57 | - | expression tag |
| Phe -56 | - | expression tag |
| Gln -55 | - | expression tag |
| Gly -54 | - | expression tag |
| Pro -53 | - | expression tag |
| Trp -46 | Met 29 | conflict |
| Ile 49 | His 124 | conflict |
| Leu 53 | - | linker |
| Ala 54 | - | linker |
| Gly 55 | - | linker |
| Ser 56 | - | linker |
| Pro 57 | - | linker |
| Gly 58 | - | linker |
| Ala 59 | - | linker |
| Arg 60 | - | linker |
| Ser 61 | - | linker |
| Ala 62 | - | linker |
| Ser 63 | - | linker |
| Ser 64 | - | linker |
| Leu 65 | - | linker |
| Ala 66 | - | linker |
| Leu 67 | - | linker |
| Ala 68 | - | linker |
| Ile 69 | - | linker |
| Ala 70 | - | linker |
| Ile 71 | - | linker |
| Thr 72 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Leu 359 | - | insertion |
| Glu 360 | - | insertion |
| Val 361 | - | insertion |
| Leu 362 | - | insertion |
| Phe 363 | - | insertion |
| Gln 364 | - | insertion |
| Gly 365 | - | insertion |
| Pro 366 | - | insertion |
| Asp 407 | - | expression tag |
| Ile 408 | - | expression tag |
| His 409 | - | expression tag |
| His 410 | - | expression tag |
| His 411 | - | expression tag |
| His 412 | - | expression tag |
| His 413 | - | expression tag |
| His 414 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 141071.1 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1187.8 | |
| All* | Total formula weight | 142258.9 |
