7T2G
CryoEM structure of mu-opioid receptor - Gi protein complex bound to mitragynine pseudoindoxyl (MP)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40415.0 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 344 | 37671.1 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
3 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
4 | R | Soluble cytochrome b562,Mu-type opioid receptor | polymer | 488 | 55123.8 | 1 | UniProt (P0ABE7) UniProt (P42866) Pfam (PF07361) Pfam (PF00001) In PDB | Escherichia coli | Cytochrome b-562,M-OR-1,MOR-1 |
5 | R | Mitragynine pseudoindoxyl | non-polymer | 414.5 | 1 | Chemie (EIG) | |||
6 | R | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
7 | water | water | 18.0 | 8 | Chemie (HOH) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: -52 - 52 (UniProt: P0ABE7)
R: 73 - 406 (UniProt: P42866)
PDB | External Database | Details |
---|---|---|
Pro -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Cys -73 | - | expression tag |
Leu -72 | - | expression tag |
Val -71 | - | expression tag |
Phe -70 | - | expression tag |
Ala -69 | - | expression tag |
Asp -68 | - | expression tag |
Tyr -67 | - | expression tag |
Lys -66 | - | expression tag |
Asp -65 | - | expression tag |
Asp -64 | - | expression tag |
Asp -63 | - | expression tag |
Asp -62 | - | expression tag |
Ala -61 | - | expression tag |
Leu -60 | - | expression tag |
Glu -59 | - | expression tag |
Val -58 | - | expression tag |
Leu -57 | - | expression tag |
Phe -56 | - | expression tag |
Gln -55 | - | expression tag |
Gly -54 | - | expression tag |
Pro -53 | - | expression tag |
Trp -46 | Met 29 | conflict |
Ile 49 | His 124 | conflict |
Leu 53 | - | linker |
Ala 54 | - | linker |
Gly 55 | - | linker |
Ser 56 | - | linker |
Pro 57 | - | linker |
Gly 58 | - | linker |
Ala 59 | - | linker |
Arg 60 | - | linker |
Ser 61 | - | linker |
Ala 62 | - | linker |
Ser 63 | - | linker |
Ser 64 | - | linker |
Leu 65 | - | linker |
Ala 66 | - | linker |
Leu 67 | - | linker |
Ala 68 | - | linker |
Ile 69 | - | linker |
Ala 70 | - | linker |
Ile 71 | - | linker |
Thr 72 | - | linker |
PDB | External Database | Details |
---|---|---|
Leu 359 | - | insertion |
Glu 360 | - | insertion |
Val 361 | - | insertion |
Leu 362 | - | insertion |
Phe 363 | - | insertion |
Gln 364 | - | insertion |
Gly 365 | - | insertion |
Pro 366 | - | insertion |
Asp 407 | - | expression tag |
Ile 408 | - | expression tag |
His 409 | - | expression tag |
His 410 | - | expression tag |
His 411 | - | expression tag |
His 412 | - | expression tag |
His 413 | - | expression tag |
His 414 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 141071.1 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 1187.8 | |
All* | Total formula weight | 142258.9 |