7T1U

Crystal structure of a superbinder Src SH2 domain (sSrcF) in complex with a high affinity phosphopeptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Proto-oncogene tyrosine-protein kinase Src	polymer	111	12659.2	2	UniProt (P12931) Pfam (PF00017)	Homo sapiens (human)	Proto-oncogene c-Src,pp60c-src,p60-Src
2	C, D (D, E)	Synthetic phosphopeptide	polymer	7	986.9	2		synthetic construct
3	E, F, G, H (A, B, E)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
4	I, J, K, L (A, B, D, E)	water	water		18.0	18	Chemie (HOH)

Sequence modifications

A, B: 147 - 250 (UniProt: P12931)

PDB	External Database	Details
Gly 142	-	expression tag
Gly 143	-	expression tag
Ala 144	-	expression tag
Pro 145	-	expression tag
Trp 146	-	expression tag
Gly 180	Ser 180	engineered mutation
Gln 181	Glu 181	engineered mutation
-	Thr 182	deletion
Ser 182	Thr 183	engineered mutation
Gln 183	Lys 184	engineered mutation
Pro 184	Gly 185	engineered mutation
Asp 185	Ala 186	engineered mutation
Val 187	Cys 188	engineered mutation
Ile 205	Lys 206	engineered mutation
Arg 251	-	expression tag
Ala 252	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	27292.2
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	27553.8

*Water molecules are not included.