7SR8

Molecular mechanism of the the wake-promoting agent TAK-925

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	a modified Guanine nucleotide-binding protein G(q) subunit alpha	polymer	238	27638.5	1		Homo sapiens
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (Human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	82	9137.5	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (E)	scFv16	polymer	259	27784.9	1		Homo sapiens
5	E (R)	Hypocretin receptor type 2	polymer	407	46848.0	1	UniProt (Q548Y0) Pfam (PF00001) Pfam (PF03827) UniProt (by SIFTS) (O43614)	Homo sapiens (Human)	Orexin receptor type 2
6	F (R)	methyl (2R,3S)-3-[(methanesulfonyl)amino]-2-({[(1s,4S)-4-phenylcyclohexyl]oxy}methyl)piperidine-1-carboxylate	non-polymer		424.6	1	Chemie (A6F)
7	G, H, I, J (R)	OLEIC ACID	non-polymer		282.5	4	Chemie (OLA)

Sequence modifications

G: 2 - 71 (UniProt: P59768)

PDB	External Database	Details
Met -10	-	expression tag
Gly -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag

R: 3 - 408 (UniProt: Q548Y0)

PDB	External Database	Details
Met 2	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	148825.8
Non-Polymers*	Number of molecules	5
	Total formula weight	1554.4
All*	Total formula weight	150380.2

*Water molecules are not included.