7SR5

Single chain trimer HLA-A*02:01 (Y108C, A163C) with Wilms tumor protein peptide RMFPNAPYL

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Wilms tumor protein peptide,Beta-2-microglobulin,MHC class I antigen chimera	polymer	424	47683.5	2	UniProt (P19544) UniProt (P16213) UniProt (A0A678ZGP6) Pfam (PF07654) Pfam (PF00129) UniProt (by SIFTS) (A0A6S7XV52)	Homo sapiens (Human)	WT33
2	B, D (B, D)	VHH	polymer	116	12536.8	2		Lama glama
3	E, F, G, H (A, B, C, D)	water	water		18.0	225	Chemie (HOH)

Sequence modifications

A, C: 1 - 9 (UniProt: P19544)

PDB	External Database	Details
Gly 10	-	linker
Gly 11	-	linker
Gly 12	-	linker
Gly 13	-	linker
Ser 14	-	linker
Gly 15	-	linker
Gly 16	-	linker
Gly 17	-	linker
Gly 18	-	linker
Ser 19	-	linker
Gly 20	-	linker
Gly 21	-	linker
Gly 22	-	linker
Gly 23	-	linker
Ser 24	-	linker

A, C: 25 - 123 (UniProt: P16213)

PDB	External Database	Details
Gly 124	-	linker
Gly 125	-	linker
Gly 126	-	linker
Gly 127	-	linker
Ser 128	-	linker
Gly 129	-	linker
Gly 130	-	linker
Gly 131	-	linker
Gly 132	-	linker
Ser 133	-	linker
Gly 134	-	linker
Gly 135	-	linker
Gly 136	-	linker
Gly 137	-	linker
Ser 138	-	linker
Gly 139	-	linker
Gly 140	-	linker
Gly 141	-	linker
Gly 142	-	linker
Ser 143	-	linker

A, C: 144 - 418 (UniProt: A0A678ZGP6)

PDB	External Database	Details
Cys 227	Tyr 108	engineered mutation
Cys 282	Ala 163	engineered mutation
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	120440.6
All*	Total formula weight	120440.6

*Water molecules are not included.