7SR5

Single chain trimer HLA-A*02:01 (Y108C, A163C) with Wilms tumor protein peptide RMFPNAPYL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-06-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	117.476, 117.476, 261.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.740 - 2.350
R-factor	0.2304
Rwork	0.229
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7sr0
RMSD bond length	0.002
RMSD bond angle	0.479
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	76503	7530
<I/σ(I)>	16.8
Completeness [%]	99.4
Redundancy	7.1
CC(1/2)	0.997	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	200 mM NH4Citrate, 14.5% PEG 3350