7SQO

Structure of the orexin-2 receptor(OX2R) bound to TAK-925, Gi and scFv16

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Orexin receptor type 2	polymer	444	50733.3	1	UniProt (O43614) Pfam (PF00001) Pfam (PF03827) In PDB	Homo sapiens (human)	Ox-2-R,Ox2-R,Ox2R,Hypocretin receptor type 2
2	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40459.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	354	39151.9	1	UniProt (P62871) Pfam (PF00400) In PDB	Bos taurus (cattle)	Transducin beta chain 1
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (cattle)	G gamma-I
5	E	scFv-16	polymer	247	26277.3	1		synthetic construct
6	R	OLEIC ACID	non-polymer		282.5	4	Chemie (OLA)
7	R	methyl (2R,3S)-3-[(methanesulfonyl)amino]-2-({[(1s,4S)-4-phenylcyclohexyl]oxy}methyl)piperidine-1-carboxylate	non-polymer		424.6	1	Chemie (A6F)

Sequence modifications

R: 1 - 444 (UniProt: O43614)

PDB	External Database	Details
Val 308	Ile 308	conflict

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asp 206	Ser 206	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P62871)

PDB	External Database	Details
Met -13	-	initiating methionine
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	164185.2
Non-Polymers*	Number of molecules	5
	Total formula weight	1554.4
All*	Total formula weight	165739.6

*Water molecules are not included.