7SNY

2.10A Resolution Structure of NanoBiT Complementation Reporter Large Subunit LgBiT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Oplophorus-luciferin 2-monooxygenase catalytic subunit	polymer	165	18572.1	1	UniProt (Q9GV45)	Oplophorus gracilirostris (Luminous shrimp)	19kOLase
2	B (A)	water	water		18.0	32	Chemie (HOH)

Sequence modifications

A: 1 - 156 (UniProt: Q9GV45)

PDB	External Database	Details
Met -1	-	initiating methionine
Val 0	-	expression tag
Glu 4	Ala 31	engineered mutation
Glu 11	Gln 38	engineered mutation
Ala 15	Gly 42	engineered mutation
Leu 18	Gln 45	engineered mutation
Val 27	Leu 54	engineered mutation
Leu 31	Phe 58	engineered mutation
Asn 33	Ala 60	engineered mutation
Ala 35	Gly 62	engineered mutation
Arg 43	Lys 70	engineered mutation
Ile 44	Val 71	engineered mutation
Arg 46	Leu 73	engineered mutation
Ala 51	Gly 78	engineered mutation
Ile 54	Ala 81	engineered mutation
Ala 67	Gly 94	engineered mutation
Asp 68	Phe 95	engineered mutation
Ala 71	Gly 98	engineered mutation
Gln 72	Leu 99	engineered mutation
Glu 75	Met 102	engineered mutation
Val 76	Ile 103	engineered mutation
Val 90	Ile 117	engineered mutation
Pro 93	His 120	engineered mutation
Leu 107	Ile 134	engineered mutation
Asn 108	Asp 135	engineered mutation
Glu 115	Pro 142	engineered mutation
Lys 124	Gln 151	engineered mutation
Ile 138	Tyr 165	engineered mutation
Thr 144	Asn 171	engineered mutation
Met 149	Leu 176	engineered mutation
Ser 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
His 162	-	expression tag
His 163	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	18572.1
All*	Total formula weight	18572.1

*Water molecules are not included.