7SNY

2.10A Resolution Structure of NanoBiT Complementation Reporter Large Subunit LgBiT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	62.342, 62.342, 104.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.730 - 2.100
R-factor	0.2234
Rwork	0.221
R-free	0.27690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ibo
Data reduction software	XDS
Data scaling software	Aimless (0.5.1)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (dev_4289)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.630	40.630	2.160
High resolution limit [Å]	2.100	8.910	2.100
Rmerge	0.083	0.036	1.793
Rmeas	0.087	0.038	1.862
Rpim	0.024	0.012	0.499
Total number of observations	162972	2316	13536
Number of reflections	12596	211	998
<I/σ(I)>	17.6	59.8	1.9
Completeness [%]	99.7	99.3	99.5
Redundancy	12.9	11	13.6
CC(1/2)	0.999	0.999	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	15% (v/v) 2-methyl-2,4-pentanediol, 0.1 M sodium acetate, 2% (w/v) PEG 4000