7SNX

1.70A Resolution Structure of NanoBiT Complementation Reporter Complex of LgBit and SmBiT Subunits

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Oplophorus-luciferin 2-monooxygenase catalytic subunit	polymer	163	18130.6	1	UniProt (Q9GV45)	Oplophorus gracilirostris (Luminous shrimp)	19kOLase
2	B (B)	Oplophorus-luciferin 2-monooxygenase catalytic subunit: C-terminal Peptide (11-mer)	polymer	12	1366.6	1	UniProt (Q9GV45)	Oplophorus gracilirostris	19kOLase
3	C (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	D, E (A, B)	water	water		18.0	194	Chemie (HOH)

Sequence modifications

A: 1 - 156 (UniProt: Q9GV45)

PDB	External Database	Details
Ser -4	-	expression tag
Asp -3	-	expression tag
Asn -2	-	expression tag
Met -1	-	expression tag
Val 0	-	expression tag
Glu 4	Ala 31	engineered mutation
Glu 11	Gln 38	engineered mutation
Ala 15	Gly 42	engineered mutation
Leu 18	Gln 45	engineered mutation
Val 27	Leu 54	engineered mutation
Leu 31	Phe 58	engineered mutation
Asn 33	Ala 60	engineered mutation
Ala 35	Gly 62	engineered mutation
Arg 43	Lys 70	engineered mutation
Ile 44	Val 71	engineered mutation
Arg 46	Leu 73	engineered mutation
Ala 51	Gly 78	engineered mutation
Ile 54	Ala 81	engineered mutation
Ala 67	Gly 94	engineered mutation
Asp 68	Phe 95	engineered mutation
Ala 71	Gly 98	engineered mutation
Gln 72	Leu 99	engineered mutation
Glu 75	Met 102	engineered mutation
Val 76	Ile 103	engineered mutation
Val 90	Ile 117	engineered mutation
Pro 93	His 120	engineered mutation
Leu 107	Ile 134	engineered mutation
Asn 108	Asp 135	engineered mutation
Glu 115	Pro 142	engineered mutation
Lys 124	Gln 151	engineered mutation
Ile 138	Tyr 165	engineered mutation
Thr 144	Asn 171	engineered mutation
Met 149	Leu 176	engineered mutation
Ser 157	-	expression tag
Ala 158	-	expression tag

B: 158 - 168 (UniProt: Q9GV45)

PDB	External Database	Details
Ace 157	-	acetylation
Tyr 161	Trp 188	engineered mutation
Phe 164	Cys 191	engineered mutation
Glu 166	Asn 193	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	19497.2
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	19589.2

*Water molecules are not included.