7SNX
1.70A Resolution Structure of NanoBiT Complementation Reporter Complex of LgBit and SmBiT Subunits
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.418, 73.418, 101.109 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.710 - 1.700 |
R-factor | 0.1578 |
Rwork | 0.157 |
R-free | 0.17850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ibo |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX (dev_4289) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.180 | 46.180 | 1.730 |
High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
Rmerge | 0.116 | 0.033 | 1.307 |
Total number of observations | 214426 | 1590 | 11527 |
Number of reflections | 31166 | 273 | 1627 |
<I/σ(I)> | 12.2 | 37.3 | 1.6 |
Completeness [%] | 100.0 | 99.5 | 100 |
Redundancy | 6.9 | 5.8 | 7.1 |
CC(1/2) | 0.998 | 0.999 | 0.535 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 1.5 M Na/K Phosphate, 4 (v/v) % 1,3-propanediol |