7SJ2

N-acetylglucosamine-1-phosphotransferase (GNPT) alpha and beta subunits (GNPTAB) catalytic domain, from zebrafish, in complex with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) and magnesium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	N-acetylglucosamine-1-phosphotransferase subunit alpha,N-acetylglucosamine-1-phosphotransferase (GNPT) alpha (GNPTAB) catalytic domain,N-acetylglucosamine-1-phosphotransferase subunit beta	polymer	495	58363.0	2	UniProt (Q5RGJ8) UniProt (Q54MP1) Pfam (PF17101) Pfam (PF11380) Pfam (PF17102) Pfam (PF17103)	Danio rerio (Zebrafish Brachydanio rerio)
2	C (C)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
3	D, G (D, G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	2	In PDB GlyTouCan (G21290RB)
4	E (E)	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	1	In PDB GlyTouCan (G86851RC)
5	F (F)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
6	H, I, N, O (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
7	J, P (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
8	K, L, Q, R (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
9	M, S (A, B)	URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE	non-polymer		607.4	2	Chemie (UD1)
10	T, U (A, B)	water	water		18.0	768	Chemie (HOH)

Sequence modifications

A, B: 44 - 91 (UniProt: Q5RGJ8)

PDB	External Database	Details
Asp 34	-	expression tag
Arg 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
His 40	-	expression tag
His 41	-	expression tag
Gly 42	-	expression tag
Ser 43	-	expression tag

A, B: 92 - 130 (UniProt: Q54MP1)
A, B: 131 - 248 (UniProt: Q5RGJ8)
A, B: 249 - 528 (UniProt: Q5RGJ8)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	116726.0
Branched	Number of molecules	5
	Total formula weight	3005.8
Non-Polymers*	Number of molecules	12
	Total formula weight	2276.9
All*	Total formula weight	122008.7

*Water molecules are not included.