7SJ2

N-acetylglucosamine-1-phosphotransferase (GNPT) alpha and beta subunits (GNPTAB) catalytic domain, from zebrafish, in complex with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) and magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	94.542, 87.142, 108.369
Unit cell angles	90.00, 111.86, 90.00

Refinement procedure

Resolution	47.420 - 2.300
R-factor	0.1616
Rwork	0.160
R-free	0.20180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s6n
RMSD bond length	0.003
RMSD bond angle	0.615
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	66254	2852
<I/σ(I)>	39.1
Completeness [%]	91.0
Redundancy	6.9
CC(1/2)	1.000	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	Crystallized in PEG 5000 MME, Tris pH 8.5. Soaked in PEG 3350, Tris 7.5, UDP-GlcNAc and MgCl2. Cryoprotected with glycerol.