7SHT

Structure of a partially disrupted IgE high affinity receptor complex bound to an omalizumab variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	High affinity immunoglobulin epsilon receptor subunit alpha	polymer	197	22541.8	1	UniProt (P12319) Pfam (PF13927) Pfam (PF13895)	Homo sapiens (Human)	Fc-epsilon RI-alpha,FcERI,IgE Fc receptor subunit alpha
2	B, C (B, D)	Immunoglobulin heavy constant epsilon	polymer	322	35773.2	2	UniProt (P01854) Pfam (PF07654)	Homo sapiens (Human)	Ig epsilon chain C region,Ig epsilon chain C region ND
3	D, E (H, J)	clone_7 Variable fragment heavy chain	polymer	123	13451.8	2		Homo sapiens
4	F, G (K, L)	clone_7 Variable fragment light chain	polymer	134	14641.9	2		Homo sapiens
5	H (C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
6	I (E)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
7	J, K (F, G)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	2	In PDB GlyTouCan (G21381MC)
8	L, M, N (A, H, J)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)

Sequence modifications

A: 5 - 172 (UniProt: P12319)

PDB	External Database	Details
Ala -24	-	expression tag
Pro -23	-	expression tag
Met -22	-	expression tag
Ala -21	-	expression tag
Glu -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Gln -16	-	expression tag
Asn -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Gly -6	-	expression tag
Gly -5	-	expression tag
Glu -4	-	expression tag
Asn -3	-	expression tag
Leu -2	-	expression tag
Tyr -1	-	expression tag
Phe 0	-	expression tag
Gln 1	-	expression tag
Gly 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
Cys 156	Trp 181	engineered mutation

B, D: 228 - 547 (UniProt: P01854)

PDB	External Database	Details
Ala 226	-	expression tag
Ser 227	-	expression tag
Cys 335	Gly 216	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	150275.5
Branched	Number of molecules	4
	Total formula weight	2832.6
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	153771.7

*Water molecules are not included.