7SEM

Structure-based design of prefusion-stabilized human metapneumovirus fusion proteins

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	MPE8 Fab heavy chain	polymer	228	23985.8	1		Homo sapiens
2	B (F)	Fusion glycoprotein F0	polymer	551	60357.6	1	UniProt (H6X1Z0) Pfam (PF00523)	Human metapneumovirus	Fusion glycoprotein F1,Fusion glycoprotein F2
3	C (C)	MPE8 Fab light chain	polymer	216	22772.1	1		Homo sapiens
4	D, E, F (F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
5	G, H, I (B, F, C)	water	water		18.0	173	Chemie (HOH)

Sequence modifications

F: 1 - 490 (UniProt: H6X1Z0)

PDB	External Database	Details
Arg 100	Gln 100	conflict
Arg 101	Ser 101	conflict
Cys 127	Thr 127	conflict
Cys 153	Asn 153	conflict
Pro 185	Ala 185	conflict
Cys 365	Thr 365	conflict
Asn 368	His 368	conflict
Cys 463	Val 463	conflict
Gly 491	-	expression tag
Gly 492	-	expression tag
Gly 493	-	expression tag
Ser 494	-	expression tag
Gly 495	-	expression tag
Tyr 496	-	expression tag
Ile 497	-	expression tag
Pro 498	-	expression tag
Glu 499	-	expression tag
Ala 500	-	expression tag
Pro 501	-	expression tag
Arg 502	-	expression tag
Asp 503	-	expression tag
Gly 504	-	expression tag
Gln 505	-	expression tag
Ala 506	-	expression tag
Tyr 507	-	expression tag
Val 508	-	expression tag
Arg 509	-	expression tag
Lys 510	-	expression tag
Asp 511	-	expression tag
Gly 512	-	expression tag
Glu 513	-	expression tag
Trp 514	-	expression tag
Val 515	-	expression tag
Leu 516	-	expression tag
Leu 517	-	expression tag
Ser 518	-	expression tag
Thr 519	-	expression tag
Phe 520	-	expression tag
Leu 521	-	expression tag
Gly 522	-	expression tag
Arg 523	-	expression tag
Ser 524	-	expression tag
Leu 525	-	expression tag
Glu 526	-	expression tag
Val 527	-	expression tag
Leu 528	-	expression tag
Phe 529	-	expression tag
Gln 530	-	expression tag
Gly 531	-	expression tag
Pro 532	-	expression tag
Gly 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
Ser 542	-	expression tag
Ala 543	-	expression tag
Trp 544	-	expression tag
Ser 545	-	expression tag
His 546	-	expression tag
Pro 547	-	expression tag
Gln 548	-	expression tag
Phe 549	-	expression tag
Glu 550	-	expression tag
Lys 551	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	107115.5
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	107779.1

*Water molecules are not included.