7SEM

Structure-based design of prefusion-stabilized human metapneumovirus fusion proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 2 1
Unit cell lengths	68.169, 45.560, 176.270
Unit cell angles	90.00, 94.21, 90.00

Refinement procedure

Resolution	43.950 - 2.200
R-factor	0.2176
Rwork	0.216
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wb0 5u68
RMSD bond length	0.008
RMSD bond angle	1.107
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.950	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	0.548
Rpim	0.057	0.542
Number of reflections	54115	4437
<I/σ(I)>	9.3	1.8
Completeness [%]	97.6
Redundancy	3.2
CC(1/2)	0.997	0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	100 nl of the complex (5.4 mg/ml) with 50 nl of reservoir solution containing 10% (v/v) isopropanol, 0.1 M HEPES pH 7.5, and 20% (w/v) PEG4000