7SD3

Cytoplasmic tail deleted HIV-1 Env bound with three 4E10 Fabs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (H, N, M)	4E10 Fab light chain	polymer	214	23292.7	3		Homo sapiens
2	B, D, F (G, K, I)	4E10 Fab heavy chain	polymer	230	24180.3	3		Homo sapiens
3	G, I, K (A, C, E)	Envelope glycoprotein gp120	polymer	481	54064.3	3	UniProt (A0A6H1VID3) Pfam (PF00516) UniProt (by SIFTS) (Q2N0S6)	HIV whole-genome vector AA1305#18	Envelope glycoprotein gp160,Glycoprotein 120,Surface protein gp120,Transmembrane protein gp41
4	H, J, L (B, D, F)	Transmembrane protein gp41	polymer	173	19800.6	3	UniProt (A0A173DX29) Pfam (PF00517) UniProt (by SIFTS) (Q2N0S6)	HIV whole-genome vector AA1305#18	Env polyprotein
5	M, O, P, R, S... (J, O, P, R, S...)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	6	In PDB GlyTouCan (G15407YE)
6	N, Q, T (L, Q, T)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
7	AA, AB, BA, BB, CA... (A, E, F, B, C...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	36	Chemie (NAG)

Sequence modifications

A, C, E: 31 - 506 (UniProt: A0A6H1VID3)

PDB	External Database	Details
Ser 374	Tyr 373	conflict
Cys 499	Ala 498	conflict
Arg 507	-	expression tag
Arg 508	-	expression tag
Arg 509	-	expression tag
Arg 510	-	expression tag
Arg 511	-	expression tag

B, D, F: 512 - 684 (UniProt: A0A173DX29)

PDB	External Database	Details
Pro 559	Ile 556	conflict
Cys 605	Thr 602	conflict
Leu 684	Ile 681	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	364013.4
Branched	Number of molecules	9
	Total formula weight	4792.5
Non-Polymers*	Number of molecules	36
	Total formula weight	7963.5
All*	Total formula weight	376769.3

*Water molecules are not included.