7S8L

CryoEM structure of Gq-coupled MRGPRX2 with peptide agonist Cortistatin-14

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Mas-related G-protein coupled receptor member X2	polymer	331	37147.0	1	UniProt (Q96LB1) Pfam (PF00001) In PDB	Homo sapiens (Human)
2	A	Cortistatin 14	polymer	14	1726.0	1	Pfam (PF03002) UniProt (by SIFTS) (O00230) In PDB	Homo sapiens
3	B	Gs-mini-Gq chimera	polymer	246	28084.8	1		Homo sapiens (Human)
4	C	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37728.2	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
5	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
6	E	scFv16	polymer	257	27409.6	1		Mus musculus (Mouse)

Sequence modifications

R: 2 - 330 (UniProt: Q96LB1)

PDB	External Database	Details
Gly 0	-	expression tag
Pro 1	-	expression tag

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	139956.7
All*	Total formula weight	139956.7

*Water molecules are not included.