7S6C

CryoEM structure of modular PKS holo-Lsd14 stalled at the condensation step and bound to antibody fragment 1B2, composite structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion	polymer	1649	174085.7	4	UniProt (Q03132) UniProt (B6ZK67) Pfam (PF08990) Pfam (PF00109) Pfam (PF02801) Pfam (PF16197) Pfam (PF00698) Pfam (PF18369) Pfam (PF08659) Pfam (PF00550)	Saccharopolyspora erythraea	DEBS 2,6-deoxyerythronolide B synthase II,Erythronolide synthase,ORF B
2	E, H (E, H)	Fab 1B2 heavy chain	polymer	249	26447.6	2		Homo sapiens (Human)
3	F, G (F, G)	Fab 1B2 light chain	polymer	236	25715.8	2		Homo sapiens (Human)
4	I (C)	2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE	non-polymer		507.2	1	Chemie (ATR)
5	J (D)	4'-PHOSPHOPANTETHEINE	non-polymer		358.3	1	Chemie (PNS)

Sequence modifications

A, B, C, D: 9 - 37 (UniProt: Q03132)

PDB	External Database	Details
Met 7	-	initiating methionine
Val 8	-	expression tag

A, B, C, D: 38 - 1647 (UniProt: B6ZK67)

PDB	External Database	Details
Leu 1648	-	expression tag
Glu 1649	-	expression tag
His 1650	-	expression tag
His 1651	-	expression tag
His 1652	-	expression tag
His 1653	-	expression tag
His 1654	-	expression tag
His 1655	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	800669.8
Non-Polymers*	Number of molecules	2
	Total formula weight	865.5
All*	Total formula weight	801535.4

*Water molecules are not included.