7S6C
CryoEM structure of modular PKS holo-Lsd14 stalled at the condensation step and bound to antibody fragment 1B2, composite structure
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004312 | molecular_function | fatty acid synthase activity |
| A | 0004315 | molecular_function | 3-oxoacyl-[acyl-carrier-protein] synthase activity |
| A | 0006633 | biological_process | fatty acid biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016746 | molecular_function | acyltransferase activity |
| A | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| A | 0017000 | biological_process | antibiotic biosynthetic process |
| A | 0031177 | molecular_function | phosphopantetheine binding |
| A | 0033068 | biological_process | macrolide biosynthetic process |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004312 | molecular_function | fatty acid synthase activity |
| B | 0004315 | molecular_function | 3-oxoacyl-[acyl-carrier-protein] synthase activity |
| B | 0006633 | biological_process | fatty acid biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016746 | molecular_function | acyltransferase activity |
| B | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| B | 0017000 | biological_process | antibiotic biosynthetic process |
| B | 0031177 | molecular_function | phosphopantetheine binding |
| B | 0033068 | biological_process | macrolide biosynthetic process |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004312 | molecular_function | fatty acid synthase activity |
| C | 0004315 | molecular_function | 3-oxoacyl-[acyl-carrier-protein] synthase activity |
| C | 0006633 | biological_process | fatty acid biosynthetic process |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016746 | molecular_function | acyltransferase activity |
| C | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| C | 0017000 | biological_process | antibiotic biosynthetic process |
| C | 0031177 | molecular_function | phosphopantetheine binding |
| C | 0033068 | biological_process | macrolide biosynthetic process |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004312 | molecular_function | fatty acid synthase activity |
| D | 0004315 | molecular_function | 3-oxoacyl-[acyl-carrier-protein] synthase activity |
| D | 0006633 | biological_process | fatty acid biosynthetic process |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016746 | molecular_function | acyltransferase activity |
| D | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| D | 0017000 | biological_process | antibiotic biosynthetic process |
| D | 0031177 | molecular_function | phosphopantetheine binding |
| D | 0033068 | biological_process | macrolide biosynthetic process |
Functional Information from PROSITE/UniProt
| site_id | PS00012 |
| Number of Residues | 16 |
| Details | PHOSPHOPANTETHEINE Phosphopantetheine attachment site. DLGFDSLMAVELRNRI |
| Chain | Residue | Details |
| A | ASP1521-ILE1536 |
| site_id | PS00606 |
| Number of Residues | 17 |
| Details | KS3_1 Ketosynthase family 3 (KS3) active site signature. GPAvtIDtACSSSlvAL |
| Chain | Residue | Details |
| A | GLY201-LEU217 |
| site_id | PS00290 |
| Number of Residues | 7 |
| Details | IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YICNVNH |
| Chain | Residue | Details |
| E | TYR201-HIS207 | |
| F | TYR214-HIS220 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Acyl-thioester intermediate; for beta-ketoacyl synthase 1 activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU01348","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17719492","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {"description":"For beta-ketoacyl synthase 1 activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU01348","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Acyl-ester intermediate; for acyltransferase 1 activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU10022","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Site: {"description":"Important for substrate specificity of the beta-ketoacyl synthase 1","evidences":[{"source":"PubMed","id":"17719492","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Active site: {"description":"For C2-type beta-ketoacyl reductase 1 and probable racemase activity","evidences":[{"source":"UniProtKB","id":"Q9ZGI4","evidenceCode":"ECO:0000250"},{"source":"PubMed","id":"21570406","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"source":"PROSITE-ProRule","id":"PRU00258","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
