7S3V

Structure of HsKYNase_66, an evolved variant of human kynureninase with greatly increased activity towards kynurenine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Kynureninase	polymer	465	53009.8	2	UniProt (Q16719) Pfam (PF22580)	Homo sapiens (Human)	L-kynurenine hydrolase

Sequence modifications

A, B: 1 - 465 (UniProt: Q16719)

PDB	External Database	Details
Asp 67	Asn 67	engineered mutation
Asn 72	Leu 72	engineered mutation
Ile 99	Ala 99	engineered mutation
Trp 102	His 102	engineered mutation
Phe 103	Glu 103	engineered mutation
Glu 104	Val 104	engineered mutation
Asp 106	Lys 106	engineered mutation
Ser 107	Arg 107	engineered mutation
His 111	Thr 111	engineered mutation
Tyr 112	Gly 112	engineered mutation
Val 132	Ala 132	engineered mutation
Thr 136	Ala 136	engineered mutation
Ile 189	Met 189	engineered mutation
Ile 223	Val 223	engineered mutation
Tyr 225	Phe 225	engineered mutation
Gly 274	Ser 274	engineered mutation
Ser 280	Ala 280	engineered mutation
Ser 281	Gly 281	engineered mutation
Pro 282	Ala 282	engineered mutation
Cys 331	Ile 331	engineered mutation
Thr 333	Asn 333	engineered mutation
Ile 341	Ser 341	engineered mutation
Leu 405	Ile 405	engineered mutation
Asn 408	Ser 408	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	106019.7
All*	Total formula weight	106019.7

*Water molecules are not included.