7S3V

Structure of HsKYNase_66, an evolved variant of human kynureninase with greatly increased activity towards kynurenine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006569	biological_process	tryptophan catabolic process
A	0009435	biological_process	NAD biosynthetic process
A	0016787	molecular_function	hydrolase activity
A	0019363	biological_process	pyridine nucleotide biosynthetic process
A	0019441	biological_process	tryptophan catabolic process to kynurenine
A	0019805	biological_process	quinolinate biosynthetic process
A	0030170	molecular_function	pyridoxal phosphate binding
A	0030429	molecular_function	kynureninase activity
A	0034341	biological_process	response to type II interferon
A	0034354	biological_process	'de novo' NAD biosynthetic process from tryptophan
A	0034516	biological_process	response to vitamin B6
A	0042803	molecular_function	protein homodimerization activity
A	0043420	biological_process	anthranilate metabolic process
A	0097053	biological_process	L-kynurenine catabolic process
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0006569	biological_process	tryptophan catabolic process
B	0009435	biological_process	NAD biosynthetic process
B	0016787	molecular_function	hydrolase activity
B	0019363	biological_process	pyridine nucleotide biosynthetic process
B	0019441	biological_process	tryptophan catabolic process to kynurenine
B	0019805	biological_process	quinolinate biosynthetic process
B	0030170	molecular_function	pyridoxal phosphate binding
B	0030429	molecular_function	kynureninase activity
B	0034341	biological_process	response to type II interferon
B	0034354	biological_process	'de novo' NAD biosynthetic process from tryptophan
B	0034516	biological_process	response to vitamin B6
B	0042803	molecular_function	protein homodimerization activity
B	0043420	biological_process	anthranilate metabolic process
B	0097053	biological_process	L-kynurenine catabolic process

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03017

Chain	Residue	Details
A	LEU137
A	SER221
B	LEU137
B	SER221

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03017, ECO:0000269|PubMed:17300176

Chain	Residue	Details
A	THR138
B	TYR275
B	TRP305
B	THR333
A	ASP250
A	HIS253
A	TYR275
A	TRP305
A	THR333
B	THR138
B	ASP250
B	HIS253

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING:

Chain	Residue	Details
A	PHE165
B	PHE165

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:22814378

Chain	Residue	Details
A	MET1
B	MET1

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: N6-(pyridoxal phosphate)lysine

Chain	Residue	Details
A	LLP276
B	LLP276

---