7S1Y
Cryo-EM structure of human NKCC1 K289NA492E bound with bumetanide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Solute carrier family 12 member 2 | polymer | 1215 | 131841.6 | 2 | UniProt (P55011) Pfam (PF08403) Pfam (PF00324) Pfam (PF03522) | Homo sapiens (Human) | Basolateral Na-K-Cl symporter,Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 |
| 2 | C, F (A, B) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 3 | D, G (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 4 | E, H (A, B) | 3-(butylamino)-4-phenoxy-5-sulfamoylbenzoic acid | non-polymer | 364.4 | 2 | Chemie (82U) |
Sequence modifications
A, B: 3 - 1212 (UniProt: P55011)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Met 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Ser 2 | - | expression tag |
| - | Gly 192 | deletion |
| Asn 289 | Lys 289 | engineered mutation |
| Glu 492 | Ala 492 | engineered mutation |
| Leu 518 | Ile 518 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 263683.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 877.9 | |
| All* | Total formula weight | 264561.1 |
