7S0G

Isoproterenol bound beta1 adrenergic receptor in complex with heterotrimeric Gi/s chimera protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Beta1-Adrenergic Receptor	polymer	508	57958.7	1	Pfam (PF00001) UniProt (by SIFTS) (P07700) In PDB	Meleagris gallopavo
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	339	37285.7	1	UniProt (P62871) Pfam (PF00400) In PDB	Bos taurus (Bovine)	Transducin beta chain 1
3	A	Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short chimera	polymer	379	43370.3	1	UniProt (P10824) UniProt (P04896) Pfam (PF00503) In PDB	Rattus norvegicus (Rat)	Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (Bovine)	G gamma-I
5	R	ISOPRENALINE	non-polymer		211.3	1	Chemie (5FW)

Sequence modifications

A: 1 - 343 (UniProt: P10824)

PDB	External Database	Details
Met -24	-	initiating methionine
Gly -23	-	expression tag
Ser -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Val -9	-	expression tag
Leu -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Pro -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 203	Gly 203	engineered mutation

A: 344 - 354 (UniProt: P04896)
G: 1 - 71 (UniProt: P63212)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	146459.8
Non-Polymers*	Number of molecules	1
	Total formula weight	211.3
All*	Total formula weight	146671.1

*Water molecules are not included.