7S0F

Isoproterenol bound beta1 adrenergic receptor in complex with heterotrimeric Gi protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Beta1-Adrenergic Receptor	polymer	508	57958.7	1	Pfam (PF00001) UniProt (by SIFTS) (P07700)	Meleagris gallopavo
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	339	37285.7	1	UniProt (P62871) Pfam (PF00400)	Bos taurus (Bovine)	Transducin beta chain 1
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1,	polymer	379	43147.1	1	UniProt (P10824) Pfam (PF00503)	Rattus norvegicus (Rat)	Adenylate cyclase-inhibiting G alpha protein
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I
5	E (R)	ISOPRENALINE	non-polymer		211.3	1	Chemie (5FW)

Sequence modifications

A: 1 - 354 (UniProt: P10824)

PDB	External Database	Details
Met -24	-	initiating methionine
Gly -23	-	expression tag
Ser -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Val -9	-	expression tag
Leu -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Pro -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 203	Gly 203	engineered mutation

G: 1 - 71 (UniProt: P63212)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	146236.6
Non-Polymers*	Number of molecules	1
	Total formula weight	211.3
All*	Total formula weight	146447.8

*Water molecules are not included.