7S05

Cryo-EM structure of human GlcNAc-1-phosphotransferase A2B2 subcomplex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	N-acetylglucosamine-1-phosphotransferase subunits alpha/beta	polymer	1179	134787.5	2	UniProt (Q3T906) Pfam (PF17101) Pfam (PF18440) Pfam (PF11380) Pfam (PF00066) Pfam (PF06464) Pfam (PF17102) Pfam (PF17103) In PDB	Homo sapiens (Human)	GlcNAc-1-phosphotransferase subunits alpha/beta,Stealth protein GNPTAB,UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta
2	C, D, E, F	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
3	B, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
4	B, A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)

Sequence modifications

B, A: 44 - 1209 (UniProt: Q3T906)

PDB	External Database	Details
Asp 31	-	expression tag
Glu 32	-	expression tag
Asp 33	-	expression tag
Gln 34	-	expression tag
Val 35	-	expression tag
Asp 36	-	expression tag
Pro 37	-	expression tag
Arg 38	-	expression tag
Leu 39	-	expression tag
Ile 40	-	expression tag
Asp 41	-	expression tag
Gly 42	-	expression tag
Lys 43	-	expression tag
Arg 923	Thr 923	conflict
Ala 924	Gly 924	conflict
Tyr 926	Gln 926	conflict
Lys 927	Leu 927	conflict
Arg 928	Lys 928	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	269575.0
Branched	Number of molecules	4
	Total formula weight	1697.6
Non-Polymers*	Number of molecules	8
	Total formula weight	1407.4
All*	Total formula weight	272680.0

*Water molecules are not included.