7RKP

The crystal structure of I38T mutant PA endonuclease (2009/H1N1/CALIFORNIA) in complex with cyclic compound SJ001034733

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Polymerase acidic protein	polymer	197	23136.3	1	UniProt (C3W5S0) In PDB	Influenza A virus (strain swl A/California/04/2009 H1N1)	RNA-directed RNA polymerase subunit P2,RNA-directed RNA polymerase subunit P2
2	A	Hexa Vinylpyrrolidone K15	non-polymer		668.9	1	Chemie (QQ4)
3	A	(5R)-5-hydroxy-16,21-dioxa-3,8,28-triazatetracyclo[20.3.1.1~2,6~.1~11,15~]octacosa-1(26),2,6(28),11(27),12,14,22,24-octaene-4,7-dione	non-polymer		421.4	1	Chemie (5Y1)
4	A	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
5	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6		water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 1 - 50 (UniProt: C3W5S0)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Thr 38	Ile 38	engineered mutation

A: 73 - 196 (UniProt: C3W5S0)

PDB	External Database	Details
Gly 51	-	linker
Gly 52	-	linker
Ser 53	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	23136.3
Non-Polymers*	Number of molecules	7
	Total formula weight	1488.4
All*	Total formula weight	24624.7

*Water molecules are not included.