7R71

Crystal Structure of the UbArk2C-UbcH5b~Ub complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin,E3 ubiquitin-protein ligase RNF165	polymer	183	20337.1	1	UniProt (P0CG48) UniProt (Q6ZSG1) Pfam (PF00240) Pfam (PF13639)	Homo sapiens (Human)	Ark2C fused to Ubiquitin
2	B (D)	Ubiquitin	polymer	76	8576.8	1	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (Human)
3	C (C)	Ubiquitin-conjugating enzyme E2 D2	polymer	152	17214.6	1	UniProt (P62837) Pfam (PF00179)	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
4	D, E (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 1 - 76 (UniProt: P0CG48)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 77	-	linker
Glu 78	-	linker
Ser 79	-	linker
Gly 80	-	linker
Ser 81	-	linker
Gly 82	-	linker
Gly 83	-	linker
Ser 84	-	linker
Gly 85	-	linker
Ser 86	-	linker

A: 255 - 346 (UniProt: Q6ZSG1)
C: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 21	Cys 21	engineered mutation
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation
Ser 107	Cys 107	engineered mutation
Ser 111	Cys 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	46128.6
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	46259.4

*Water molecules are not included.