7R71
Crystal Structure of the UbArk2C-UbcH5b~Ub complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.918400 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 53.330, 75.668, 99.379 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.591 - 2.800 |
R-factor | 0.2305 |
Rwork | 0.226 |
R-free | 0.27140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v3l |
RMSD bond length | 0.002 |
RMSD bond angle | 0.553 |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.2) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.990 | 46.990 | 2.950 |
High resolution limit [Å] | 2.796 | 8.840 | 2.800 |
Rmerge | 0.162 | 0.046 | 1.108 |
Rmeas | 0.169 | 0.048 | 1.150 |
Rpim | 0.046 | 0.014 | 0.307 |
Total number of observations | 4155 | 20393 | |
Number of reflections | 10462 | 388 | 1485 |
<I/σ(I)> | 12.4 | 37.7 | 2.6 |
Completeness [%] | 99.8 | 99.5 | 99 |
Redundancy | 13.1 | 10.7 | 13.7 |
CC(1/2) | 0.998 | 0.998 | 0.934 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.1 M MMT pH 6.0, 25% w/v PEG 1500 |